A genomic library was constructed and eleven novel polymorphic microsatellite markers developed for Santalum spicatum, a hemi-parasitic tree native to south Western Australia and highly valued for its sesquiterpene essential oil. Polymorphism was investigated in two populations of S. spicatum, one from the arid region (Laverton) and one from the semi-arid region (Pingrup). All loci were variable, all conformed to Hardy-Weinberg Equilibrium and none demonstrated Linkage Disequilibrium. The number of alleles per locus ranged from 9 to 10 averaging 9.909 for Laverton and from 2 to 10 averaging 7.818 for Pingrup.
AgroforestryGenetic diversityPolymorphismWestern Australia