Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies
- First Online:
- Cite this article as:
- Smith, K.L., Hale, J.M., Austin, J.J. et al. Conservation Genet Resour (2011) 3: 621. doi:10.1007/s12686-011-9418-3
- 90 Downloads
Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na = 9.38 and Na = 7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.