Conservation Genetics Resources

, Volume 3, Issue 4, pp 621–624

Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies

  • Katie L. Smith
  • Joshua M. Hale
  • Jeremy J. Austin
  • Jane Melville
Technical Note

DOI: 10.1007/s12686-011-9418-3

Cite this article as:
Smith, K.L., Hale, J.M., Austin, J.J. et al. Conservation Genet Resour (2011) 3: 621. doi:10.1007/s12686-011-9418-3

Abstract

Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na = 9.38 and Na = 7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.

Keywords

Hybrid zoneMicrosatelliteLitoria ewingiiLitoria paraewingiLitoria verreauxii alpinaAmphibianSouth-eastern AustraliaTree frogSpecies boundariesHybridization

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Katie L. Smith
    • 1
    • 2
    • 3
  • Joshua M. Hale
    • 1
    • 2
    • 3
  • Jeremy J. Austin
    • 4
  • Jane Melville
    • 1
  1. 1.Sciences DepartmentMuseum VictoriaMelbourneAustralia
  2. 2.Department of ZoologyUniversity of MelbourneMelbourneAustralia
  3. 3.School of BotanyUniversity of MelbourneMelbourneAustralia
  4. 4.Australian Centre for Ancient DNA, University of AdelaideAdelaideAustralia