Conservation Genetics Resources

, Volume 3, Issue 4, pp 605–607

New microsatellite markers for the assessment of fine-scale dispersal patterns in the endangered montane caddisfly Drusus discolor

  • Jutta Geismar
  • Jan Sauer
  • Peter Haase
  • Carsten Nowak
Technical Note

DOI: 10.1007/s12686-011-9414-7

Cite this article as:
Geismar, J., Sauer, J., Haase, P. et al. Conservation Genet Resour (2011) 3: 605. doi:10.1007/s12686-011-9414-7

Abstract

We present a panel of variable microsatellite markers for the montane caddisfly Drusus discolor, a rare species that we use as a model for the assessment of climate change effects on the montane freshwater community. 454 sequencing technology was used in order to generate random sequence data. Of a total of 2,648 microsatellite-carrying fragments identified, 11 variable, reproducible markers were generated and tested on 41 individuals from two populations. Allelic diversity ranged from 3 to 10 alleles and heterozygosity values varied from 0 to 0.89 (HO) and 0.12 to 0.86 (HE). A cross-species test on four caddisfly species showed that a subset of markers amplifies in related taxa.

Keywords

Short tandem repeats Landscape genetics Dispersal Aquatic insects Microsatellite development Montane biodiversity Global climate change 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jutta Geismar
    • 1
    • 2
  • Jan Sauer
    • 1
  • Peter Haase
    • 1
    • 2
  • Carsten Nowak
    • 1
    • 2
  1. 1.Biodiversity and Climate Research Centre (BiK-F)Frankfurt am MainGermany
  2. 2.Department of Limnology and ConservationSenckenberg Research Institutes and Natural History MuseumsGelnhausenGermany