Conservation Genetics Resources

, Volume 3, Issue 3, pp 523–527

Development and characterization of 10 polymorphic microsatellite loci for the blue shark, Prionace glauca, and their cross shark-species amplification

  • Seán Fitzpatrick
  • Mahmood S. Shivji
  • Demian D. Chapman
  • Paulo A. Prodöhl
Technical Note

DOI: 10.1007/s12686-011-9395-6

Cite this article as:
Fitzpatrick, S., Shivji, M.S., Chapman, D.D. et al. Conservation Genet Resour (2011) 3: 523. doi:10.1007/s12686-011-9395-6

Abstract

Ten polymorphic nuclear microsatellite loci were developed from a microsatellite enriched genomic library of the blue shark, Prionace glauca. The utility of these markers for genetic studies of this globally distributed, heavily exploited, oceanic predator was assessed by screening 120 specimens sampled from six locations throughout the species’ range. Both moderately and highly polymorphic marker loci were identified. Three to 35 alleles were found to be segregating per locus (mean 10.1) with observed heterozygosities ranging from 24 to 91%. Evaluation of the cross-species amplification of these markers across 18 additional shark species indicates that these microsatellites are potentially useful for genetic studies of other species of conservation concern.

Keywords

Blue shark Prionace glauca Carcharhinus Polymorphic Microsatellite Cross-species 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Seán Fitzpatrick
    • 1
  • Mahmood S. Shivji
    • 2
  • Demian D. Chapman
    • 2
    • 3
  • Paulo A. Prodöhl
    • 1
  1. 1.School of Biological SciencesMedical Biology Centre, Queen’s University BelfastBelfastNorthern Ireland, UK
  2. 2.Guy Harvey Research Institute and Save Our Seas Shark Centre, Oceanographic Centre, Nova South-Eastern UniversityDania BeachUSA
  3. 3.School of Marine and Atmospheric ScienceInstitute for Ocean Conservation Science, Stony Brook UniversityStony BrookUSA