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Isolation and characterization of twelve microsatellite loci of endangered Ishikawa’s frog (Odorrana ishikawae)

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Abstract

Due to an originally small distribution range and over-exploitation of primary forest, Ishikawa’s frog (Odorrana ishikawae) has been steadily declining in number. For effective conservation of this species, a greater amount of genetic information of this species is needed. Here, we isolated and characterized 12 microsatellite loci of O. ishikawae using two different methods. Loci were screened from two populations on the Amami (N = 44) and Okinawa (N = 9) Islands. The total number of alleles per locus ranged from 2 to 30, and the observed heterozygosity ranged from 0 to 0.922. Notably, high F ST values for all examined loci were observed between the two populations. Taken together, our findings suggest that these novel loci will be applicable for conservation genetic studies across varying scales.

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Authors and Affiliations

  1. Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8526, Japan

    Takeshi Igawa, Atsushi Kurabayashi & Masayuki Sumida

  2. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Masaru Okuda & Tetsuya Umino

  3. Section of Agriculture and Forest, Amami City Government, Amami, Kagoshima, 894-0048, Japan

    Shohei Oumi

  4. Biology and Ecology Group, Okinawa Prefectural Institute of Health and Environment, Nanjo, Okinawa, 901-1202, Japan

    Seiki Katsuren

Authors
  1. Takeshi Igawa
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  2. Masaru Okuda
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  3. Shohei Oumi
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  4. Seiki Katsuren
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  5. Atsushi Kurabayashi
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  6. Tetsuya Umino
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  7. Masayuki Sumida
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Corresponding author

Correspondence to Masayuki Sumida.

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Igawa, T., Okuda, M., Oumi, S. et al. Isolation and characterization of twelve microsatellite loci of endangered Ishikawa’s frog (Odorrana ishikawae). Conservation Genet Resour 3, 421–424 (2011). https://doi.org/10.1007/s12686-010-9370-7

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  • DOI: https://doi.org/10.1007/s12686-010-9370-7

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