Technical Note

Conservation Genetics Resources

, Volume 3, Issue 2, pp 243-245

First online:

Characterization of small microsatellite loci isolated in endangered Indiana bat (Myotis sodalis) for use in non-invasive sampling

  • Sara J. Oyler-McCanceAffiliated withUS Geological Survey, Fort Collins Science Center Email author 
  • , Jennifer A. FikeAffiliated withUS Geological Survey, Fort Collins Science Center

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Abstract

Primers for 10 microsatellite loci were developed specifically to amplify low quantity and quality DNA in the endangered Indiana Bat (Myotis sodalis). In a screen of 20 individuals from a population in Missouri, the 10 loci were found to have levels of variability ranging from seven to 18 alleles. No loci were found to be linked, although two loci revealed significant departures from Hardy–Weinberg equilibrium. These microsatellite loci will be applicable for population genetic analyses and for use in mark-recapture studies that utilize DNA collected non-invasively from fecal pellets, which will ultimately aid in management efforts.

Keywords

Indiana bat Microsatellite Myotis sodalis Non invasive sampling