Conservation Genetics Resources

, Volume 3, Issue 1, pp 41–43

Increased DNA amplification success of non-invasive genetic samples by successful removal of inhibitors from faecal samples collected in the field

  • Louise Hebert
  • Safi K. Darden
  • Bo Vest Pedersen
  • Torben Dabelsteen
Technical Note

DOI: 10.1007/s12686-010-9280-8

Cite this article as:
Hebert, L., Darden, S.K., Pedersen, B.V. et al. Conservation Genet Resour (2011) 3: 41. doi:10.1007/s12686-010-9280-8

Abstract

The use of non-invasive genetic sampling (NGS) is becoming increasingly important in the study of wild animal populations. Obtaining DNA from faecal samples is of particular interest because faeces can be collected without deploying sample capture devices. However, PCR amplification of DNA extracted from faeces is problematic because of high concentrations of inhibitors. Here we present a method for increasing the successful application of donor DNA extracted from faecal samples through inhibitor reduction. After standard extraction with a DNA stool kit we used a ‘Concentrated Chelex Treatment’ (CCT) that increased the amplification success from 31.7 to 61.4% of loci. Our results suggest that darker supernatant and samples with more precipitate contain more inhibitors than lighter samples and samples with little or no precipitate. We expect the use of this technique to have wide applicability within conservation biology for research and management that relies on NGS of wild animal populations.

Keywords

Non-invasive genetic sampling Faecal samples Vulpes velox Inhibitors Chelex 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Louise Hebert
    • 1
  • Safi K. Darden
    • 1
    • 2
  • Bo Vest Pedersen
    • 3
  • Torben Dabelsteen
    • 1
  1. 1.Animal Behaviour Group, Department of BiologyUniversity of CopenhagenCopenhagen ØDenmark
  2. 2.Centre for Research in Animal Behaviour, School of Psychology, Washington Singer LabsUniversity of ExeterExeterUK
  3. 3.Section for Ancient DNA, Department of BiologyUniversity of CopenhagenCopenhagen ØDenmark

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