Conservation Genetics Resources

, Volume 2, Supplement 1, pp 385–387

Characterization of nine novel microsatellites isolated from Mozambique tilapia, Oreochromis mossambicus

Technical Note

DOI: 10.1007/s12686-010-9265-7

Cite this article as:
Saju, J.M., Lee, W. & Orban, L. Conservation Genet Resour (2010) 2: 385. doi:10.1007/s12686-010-9265-7

Abstract

Nine polymorphic microsatellites were isolated from a genomic DNA library of Oreochromis mossambicus enriched with di- and tetranucleotide repeats. The three di- and six tetranucleotide-type markers were characterized in 40 Mozambique tilapia individuals collected from a local farm. The number of alleles at the nine microsatellite loci ranged from 3 to 31, with an average of 13.4/locus. The average expected heterozygosity was 0.79 (range: 0.65–0.94), whereas the average observed heterozygosity was 0.67 (range: 0.33–1). Three loci significantly deviated from Hardy–Weinberg equilibrium, presumably due to limited population size. Cross-species amplification was successful for seven markers in Oreochromis niloticus, a phylogenetically closely related species and for six markers in Pseudotropheus lombardi, an ornamental cichlid for which no microsatellites have been described to date. These markers could provide important tools for the determination of genetic diversity and population structure of O. mossambicus.

Keywords

PolymorphicHeterozygosityCross-species amplification

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Reproductive Genomics, Strategic Research ProgramTemasek Life Sciences LaboratorySingaporeSingapore
  2. 2.GenoMar AsAOsloNorway
  3. 3.Department of Biological SciencesNational University of SingaporeSingaporeSingapore