Conservation Genetics Resources

, Volume 2, Issue 1, pp 181–184

Microsatellite markers for mating system and population analyses of the spectacled caiman Caiman crocodilus (Linnaeus 1758)

  • Deyla Paula de Oliveira
  • Izeni Pires Farias
  • Boris Marioni
  • Zilca Campos
  • Tomas Hrbek
Technical Note

DOI: 10.1007/s12686-010-9221-6

Cite this article as:
de Oliveira, D.P., Farias, I.P., Marioni, B. et al. Conservation Genet Resour (2010) 2: 181. doi:10.1007/s12686-010-9221-6

Abstract

We used a microsatellite enriched library to isolate and characterize 12 di-nucleotide microsatellite loci of Caiman crocodilus. Microsatellites were characterized in populations of C. c. crocodilus from Piagaçu-Purus, Amazonas, Brazil and C. c. yacare from Cáceres, Mato Grosso, Brazil. Results of characterization were comparable between the two subspecies, with number of alleles varying from three to 20 and one to 14 per locus in C. c. crocodilus and C. c. yacare, respectively. Observed heterozygosities ranged from 0.088 to 0.816 and 0.115 to 0.833, respectively. Joint paternity exclusion (QC) was >0.999 in both subspecies, and probability of genetic identity varied from 4.631 × 10−13 in C. c. crocodilus to 2.233 × 10−8 in C. c. yacare. All loci are unlinked, and with the exception of three loci in C. c. crocodilus all loci were at Hardy–Weinberg equilibrium. The characteristics of these loci makes them an excellent tool set for the study of mating systems, and fine-scaled population structuring in the Caiman crocodilus species complex.

Keywords

Caiman crocodilus System of mating analysis Population analysis Microsatellite loci Spectacled caiman 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Deyla Paula de Oliveira
    • 1
  • Izeni Pires Farias
    • 1
  • Boris Marioni
    • 2
  • Zilca Campos
    • 3
  • Tomas Hrbek
    • 1
    • 4
  1. 1.Laboratório de Evolução e Genética Animal, Departamento de BiologiaUniversidade Federal do AmazonasManausBrazil
  2. 2.Instituto Piagaçu, Rua UZManausBrazil
  3. 3.EMBRAPA-PantanalCorumbáBrazil
  4. 4.Biology DepartmentUniversity of Puerto RicoRio Piedras, San JuanPuerto Rico

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