Conservation Genetics Resources

, Volume 2, Supplement 1, pp 279–282

Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)

  • Alison J. Peel
  • Stephen J. Rossiter
  • James L. N. Wood
  • Andrew A. Cunningham
  • David R. Sargan
Technical Note

DOI: 10.1007/s12686-010-9190-9

Cite this article as:
Peel, A.J., Rossiter, S.J., Wood, J.L.N. et al. Conservation Genet Resour (2010) 2(Suppl 1): 279. doi:10.1007/s12686-010-9190-9
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Abstract

Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3–46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.

Keywords

African bats Chiroptera Multiplex Polymorphic loci X-linked 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Alison J. Peel
    • 1
    • 2
  • Stephen J. Rossiter
    • 3
  • James L. N. Wood
    • 1
  • Andrew A. Cunningham
    • 2
  • David R. Sargan
    • 1
  1. 1.Department of Veterinary MedicineUniversity of CambridgeCambridgeUK
  2. 2.Institute of ZoologyZoological Society of LondonLondonUK
  3. 3.School of Biological and Chemical SciencesQueen Mary, University of LondonLondonUK

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