Brede, E.G., Long, P., Zefania, S. et al. Conservation Genet Resour (2010) 2(Suppl 1): 273. doi:10.1007/s12686-010-9189-2
We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species’ range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean He = 0.544 (range 0.0678–0.885), mean Ho = 0.430 (range 0.0400–0.767) and mean Na = 4.59 (range 2–10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.