Technical Note

Conservation Genetics Resources

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Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick

  • L. JungmannAffiliated withPlant Biotechnology Laboratory, EMBRAPA Beef Cattle
  • , B. B. Z. VignaAffiliated withCenter for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
  • , J. PaivaAffiliated withCenter for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
  • , A. C. B. SousaAffiliated withCenter for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
  • , C. B. do ValleAffiliated withPlant Biotechnology Laboratory, EMBRAPA Beef Cattle
  • , P. R. LabordaAffiliated withCenter for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
  • , M. I. ZucchiAffiliated withAgronomic Institute of Campinas (IAC)
  • , A. P. de SouzaAffiliated withCenter for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)Biology Institute, Plant Biology Department, University of Campinas (UNICAMP) Email author 

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Abstract

We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X–9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.

Keywords

Urochloa humidicola Koroviniagrass SSRs Taxonomy Genetic diversity