Conservation Genetics Resources

, 1:475

Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick

  • L. Jungmann
  • B. B. Z. Vigna
  • J. Paiva
  • A. C. B. Sousa
  • C. B. do Valle
  • P. R. Laborda
  • M. I. Zucchi
  • A. P. de Souza
Technical Note

DOI: 10.1007/s12686-009-9111-y

Cite this article as:
Jungmann, L., Vigna, B.B.Z., Paiva, J. et al. Conservation Genet Resour (2009) 1: 475. doi:10.1007/s12686-009-9111-y

Abstract

We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X–9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.

Keywords

Urochloa humidicolaKoroviniagrassSSRsTaxonomyGenetic diversity

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • L. Jungmann
    • 1
  • B. B. Z. Vigna
    • 2
  • J. Paiva
    • 2
  • A. C. B. Sousa
    • 2
  • C. B. do Valle
    • 1
  • P. R. Laborda
    • 2
  • M. I. Zucchi
    • 3
  • A. P. de Souza
    • 2
    • 4
  1. 1.Plant Biotechnology LaboratoryEMBRAPA Beef CattleCampo GrandeBrazil
  2. 2.Center for Molecular Biology and Genetic EngineeringUniversity of Campinas (UNICAMP)CampinasBrazil
  3. 3.Agronomic Institute of Campinas (IAC)CampinasBrazil
  4. 4.Biology Institute, Plant Biology DepartmentUniversity of Campinas (UNICAMP)CampinasBrazil