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Conservation Genetics Resources

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Development and characterization of 38 novel EST-SSRs for the sea cucumber Apostichopus japonicus

  • Wei PengAffiliated withLaboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China
  • , Zhenmin BaoAffiliated withLaboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China
  • , Huixia DuAffiliated withLaboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China
  • , Ying DongAffiliated withLiaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute
  • , Yameng LvAffiliated withLaboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China
  • , Zunchun ZhouAffiliated withLiaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute
  • , Jingjie HuAffiliated withLaboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China Email author 

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Abstract

A total of 5728 sequences from the expressed sequence tag database of sea cucumber Apostichopus japonicus were mined for microsatellite sequences. A total of 151 ESTs containing microsatellites were identified. Of 93 primer pairs designed, 56 amplified expected products and 38 showed polymorphism in 48 individuals tested. The number of alleles ranged from 2 to 10, and the observed and expected heterozygosities varied from 0.000 to 0.900 and from 0.332 to 0.884, respectively. No significant linkage disequilibrium (LD) between pairs of loci was found and 21 loci significantly deviated from the Hardy–Weinberg equilibrium (HWE).

Keywords

EST Microsatellite Sea cucumber Apostichopus japonicus