Isolation and characterization of microsatellite loci in the black pepper, Piper nigrum L. (piperaceae)

  • I. C. Menezes
  • F. W. Cidade
  • A. P. Souza
  • I. C. Sampaio
Technical Note

DOI: 10.1007/s12686-009-9051-6

Cite this article as:
Menezes, I.C., Cidade, F.W., Souza, A.P. et al. Conservation Genet Resour (2009) 1: 209. doi:10.1007/s12686-009-9051-6

Abstract

The black pepper, Piper nigrum L., which originated in Índia, is the World’s most important commercial spice. Brazil has a germplasm collection of this species preserved at the Brazilian Agricultural Research Corporation (Embrapa—Eastern Amazonia) where efforts are being made to generation information on the patterns of genetic variation and develop strategies for conservation and management of black pepper. Molecular markers of the SSR type are powerful tools for the description of material preserved in genetic resources banks, due to characteristics such as high levels of polymorphism, codominance and Mendelian segregation. Given this, we developed nine microsatellite markers from an enriched library of Piper nigrum L. Twenty varieties clonal from the Brazilian germplasm collection were analyzed, and observed and expected heterozygosity values ranged over 0.11–1.00 and 0.47–0.87, respectively. The nine microsatellite loci characterized here will contribute to studies of genetic diversity and conservation of Piper nigrum L.

Keywords

Microsatellites Piper Spices Genetic diversity 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • I. C. Menezes
    • 1
  • F. W. Cidade
    • 2
  • A. P. Souza
    • 2
  • I. C. Sampaio
    • 3
  1. 1.Empresa Brasileira de Pesquisa Agropecuária (Embrapa)-Amazônia OrientalBelémBrazil
  2. 2.Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e EvoluçãoUniversidade Estadual de CampinasCampinasBrazil
  3. 3.Instituto de Estudos Costeiros (IECOS), Universidade Federal do ParáBragançaBrazil

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