Electrocatalysis

, Volume 4, Issue 1, pp 17–23

Electrochemical Detection of Low-Molecular-Mass Biothiols in Biological Fluids at Carbon Spheres-Modified Glassy Carbon Electrodes

  • Guozhi Wu
  • Jianqing Zhou
  • Xueyue Jiang
  • Xinying Guo
  • Feng Gao
Article

DOI: 10.1007/s12678-012-0107-0

Cite this article as:
Wu, G., Zhou, J., Jiang, X. et al. Electrocatalysis (2013) 4: 17. doi:10.1007/s12678-012-0107-0

Abstract

The excellent electrocatalytic activity of a new kind of microstructured carbon material, carbon hollow spheres (CS), toward the oxidation of low-molecular-mass biological thiols including cysteine, homocysteine, and glutathione has been investigated. Comparing with conventional bare glassy carbon (GC) electrodes, an overpotential of 0.40 V is reduced at CS-modified GC electrode with electrooxidation starting at 0.0 V (vs. Ag/AgCl). Based on the electrocatalytic activity of CS, a direct electrochemical method for the determination of low-molecular-mass biothiols is proposed. The proposed method was applied to the determination of biothiols in human plasma and urine with satisfactory results.

Keywords

Electrocatalytic oxidationLow-molecular-mass biothiolsCarbon hollow spheresBiological fluids

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Guozhi Wu
    • 1
    • 2
  • Jianqing Zhou
    • 3
  • Xueyue Jiang
    • 4
  • Xinying Guo
    • 1
  • Feng Gao
    • 1
  1. 1.Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials ScienceAnhui Normal UniversityWuhuChina
  2. 2.Department of Chemistry and Food ScienceChizhou CollegeChizhouChina
  3. 3.Anhui Medical CollegeHefeiChina
  4. 4.College of Chemistry and Chemical EngineeringFuyang Teachers CollegeFuyangChina