Androgen Action in Prostate Cancer
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- Basu, S. & Tindall, D.J. HORM CANC (2010) 1: 223. doi:10.1007/s12672-010-0044-4
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Prostate cancer represents a major health problem in men worldwide. Androgens are required for the growth and maintenance of the prostate. Androgens act by binding to the androgen receptor (AR), a nuclear receptor transcription factor present in the prostate tissues. Most prostate tumors also retain their androgen dependence; therefore, androgen ablation is usually the preferred initial therapeutic approach for the treatment of advanced prostate cancer patients. This review summarizes the current information regarding the role of androgens in prostate cancer.
KeywordsAndrogenAndrogen ablation therapyProstate
Prostate cancer is the most commonly diagnosed male cancer in the Western World . Since activation of androgen receptors by androgens is required for the growth and survival of malignant prostate cells and because prostate cancer is usually androgen dependent in the beginning [2–5], androgen ablation therapy in the form of medical and or surgical castration is initially effective in inhibiting the growth of these tumors in most patients [3, 5]. However, with time, the tumor recurs in an androgen-refractory manner, presenting with a more aggressive and metastatic phenotype, which is resistant to further hormonal manipulation and therefore called castration-resistant prostate cancer [4, 5]. Since androgens play important roles in the growth and survival of prostate cancer cells [2–5], we will address the role of androgens in the development and progression of prostate cancer.
Synthesis and Metabolism of Androgens
Androgens are sex hormones, which regulate the differentiation and maturation of male reproductive organs, as well as development of male secondary characteristics . Testosterone is the primary (~90%) circulating form of androgen . The remaining androgens (~10%), which include dehydroepiandrosterone, androstenediol, and androstenedione are synthesized in the adrenal cortex, and are subsequently converted to testosterone in the peripheral tissues [4, 6]. Testosterone production is tightly controlled by the hypothalamic–pituitary–gonadal axis and is produced primarily by the Leydig cells of the testes in response to the stimulation by luteinizing hormone (LH) produced in the anterior pituitary . LH release in turn is controlled by pulsatile secretion of LH-releasing hormone (LHRH) from the hypothalamus . In addition, inhibitory signals also occur at the level of testosterone, which acts through a negative feedback loop to inhibit LHRH release by the hypothalamus and to decrease the sensitivity of the pituitary to LHRH . Circulating testosterone is bound to serum proteins, e.g., sex hormone binding globulin and albumin, leaving only 1–2% as free testosterone .
Since testosterone is lipophilic, free testosterone can easily diffuse into the target cells and, upon entry, is rapidly converted to the more potent androgen, 5-alpha dihydrotestosterone (DHT), by the enzymes 5-alpha-reductase Type I (5αR1) and 5-alpha-reductase Type II (5αR2) . Although 5αR2 has been considered traditionally to be important in the non-malignant prostate, recent reports indicate that 5αR1 has a major role in the development and progression of prostate cancer [10, 11]. A number of studies have reported that 5αR1 expression is greater in prostate cancer tissues in comparison to benign control tissues. In addition, expression and activity of 5αR2 are also decreased [11–14].
The Androgen Signaling Axis
A separate ligand-dependent, nongenotropic function of AR also exists. Androgen binding can result in AR-mediated activation of the Ras/extracellular signal-related kinase (ERK) pathway through nongenotropic activation of the c-Src nonreceptor tyrosine kinase. In some types of cells, androgen stimulation induces complex formation between AR and c-Src, as well as the estrogen receptor (ER) β-subunit. Importantly, AR-mediated cSrc activation can lead to increased cellular proliferation and protection from apoptosis [15–25].
Androgen Action in Non-malignant Prostate
The prostate is a small chestnut-sized fibromuscular glandular male accessory reproductive organ located below the bladder and surrounding the urethra . Prostatic secretions contribute to the major components of the seminal fluid that is released at ejaculation . The basal and luminal epithelium of the prostate forms the glandular acini, which are separated by a basement membrane . The epithelial cell compartment consists of basal cells lining the basement membrane, luminal columnar secretory cells, and a small number of neuroendocrine cells . The lineage and differentiation of these epithelial cells are regulated by the surrounding stroma . Androgens regulate several important functions within the epithelial compartment such as cellular proliferation, differentiation, as well as metabolic and secretory functions . AR is expressed in both the epithelial and stromal cells in adult prostate gland . Androgen deprivation induces prostate involution and loss of epithelial cells through apoptosis. However, the normal size and function of the prostate is regained through rapid proliferation and differentiation of stem cells in the basal epithelial cellular compartment following androgen stimulation . The AR functions primarily to maintain the differential secretory function of prostate epithelial cells . The androgen-dependent survival of the epithelial compartment is controlled mainly by paracrine factors modulated by the supporting AR positive stromal cells . This intricate relationship between the epithelial and stromal compartments helps to maintain the homeostasis in the prostate gland so that only 0.2% prostatic epithelial cells either proliferate or undergo apoptosis per day .
Circulating Androgens and Prostate Cancer
The direct correlation between serum androgens, especially testosterone, and risk of prostate cancer stems from the landmark studies by Huggins and Hodges , who reported regression of metastatic prostate cancer after reduction of serum testosterone levels and progression of metastatic disease and symptoms in a patient who was treated with exogenous testosterone . More recent work, however, suggests that low testosterone levels might be associated with worse clinical and pathological determinants of prostate cancer including an increased risk of prostate cancer, a worse 5-year biochemical relapse-free survival, higher Gleason score, increased percentage positive-core rate at biopsy, worse pathological stage, and increased risk of positive surgical margins . In addition, the controversy regarding finasteride in the prevention of prostate cancer and testosterone replacement therapy in hypogonadal men further indicates the uncertainty of the association between androgen and prostate cancer [30, 34, 35]. However, recent longitudinal studies of aging demonstrate an increased risk of aggressive prostate cancer among older men with higher levels of free serum testosterone . These reports have led to the development of at least two theories to explain the relationship between prostate cancer and serum testosterone levels: the “suppression theory”, which proposes that prostate cancer cells secrete an androgen inhibitor, and the “saturation theory”, which suggests that serum levels of androgens above a sufficiently low baseline are sufficient to stimulate growth of prostate cancer [30–33].
Androgen Action in Prostate Cancer
Because androgens are required for the growth and survival of malignant prostate cells, androgen ablation therapy either in the form of medical and or surgical castration is initially effective in inhibiting growth of these cancer cells in most of the patients as indicated by reduced expression of its target gene, prostate-specific antigen (PSA), and concomitant tumor regression . However, these prostate cancer relapse with a more aggressive and metastatic phenotype that is resistant to hormonal therapy and ultimately cause death of the patient . This stage of the disease is referred to as androgen-independent, androgen-refractory, androgen depletion-independent, or castration-resistant prostate cancer (CRPC) . Although CRPC prostate cancer does not respond to androgen ablation, the AR still remains a critical factor for the growth and survival of the majority of these tumors [4, 16, 27, 36, 37]. Most CRPC prostate cancer tissues have high levels of AR expression [4, 39]. In addition, the PSA gene is also expressed at this stage of the disease [4, 16]. Targeted inhibition of the AR decreases PSA expression, cell proliferation, and survival in many cell-based models of CRPC prostate cancer , thereby indicating that CRPC prostate cancer cells continue to proliferate and survive through abnormal AR activation. Thus, the AR signaling axis appears to be a valid target for therapy in this stage of the disease .
AR in Castration-Resistant Prostate Cancer
Several mechanisms can reactivate the AR in prostate cancer patients following androgen ablation, including AR gene amplification and increased AR mRNA and protein levels, AR mutations, altered expression of AR-associated coregulators, AR splice variants, and cross talk between different signal transduction pathways [40–42].
AR gene amplification has been observed in approximately 30% castration-resistant patients with recurrent prostate cancer [42–44]. However, AR gene amplification does not always lead to an increase in AR protein levels [42–44]. Higher levels of AR protein can result from gene amplification and also from increased transcription rates, or stabilization of the mRNA or protein [42–44]. AR amplification is often detected in patients who initially respond to hormone treatment and is not observed in patients with early recurrence or no initial response. The median survival time in patients with AR amplification has been reported to be twice than those without AR amplification [42–44].
Increased AR expression sensitizes prostate cancer cells to low levels of androgen and promotes progression from hormone-dependent to CRPC . Anti-androgens can also activate AR in these cells . The mechanism for this activity is attributed to an alteration in the coactivator assembly on the promoter of AR-dependent target genes . Since AR can regulate its own transcription, therefore it can also increase its synthesis by a feed-forward mechanism . These mechanisms thus play critical roles in the pathogenesis of CRPC.
AR mutations have been identified in its ligand-binding domain, as well as the amino terminus and DNA-binding domain [46–48]. These mutations are usually related to AR gain-of-function and are linked to CRPC. In addition, the majority of AR mutations, which are associated with hormone ablation therapy, are in the C-terminal ligand-binding domain. These mutations increase the sensitivity of AR to low concentrations of androgens, weak androgen precursors, progesterone, estradiol, cortisol, and even anti-androgens. A common AR mutation is at codon 877 (Thr to Ser), detected in prostate cancer tissues biopsied from patients with metastasis . A specific AR mutation at amino acid 741 (Trp to Cys or Trp to Leu) has been detected in patients receiving the anti-androgen drug, bicalutamide . Other known AR mutations that display altered ligand-binding specificity are Q798E, M715V, and H874Y . Some AR mutations outside the ligand-binding domain have also been detected in CRPC. Mutation at amino acid 255 (E255K) interferes with interaction between AR and the E3 ubiquitin ligase, CHIP. This mutant AR further exhibits increased stability and nuclear localization in the absence of ligand .
Several coactivators such as p300, FHL2, TIF2, SRC1, TIP60, and BAG-1L are present at increased levels in CRPC . The expressions of these coregulators directly or indirectly depend on the AR activity, and thereby suggest a feed-forward loop for the dysfunctional AR in CRPC. Furthermore, over-expression of these coactivators reduces the androgen requirement of AR for its activation and is considered a probable mechanism for the growth of prostate cancer after hormone ablation therapy. Furthermore, there is also a cross talk between growth factor kinase pathways and AR. Insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), interleukin-6 (IL-6) and Wnt signaling, oncostatin (OSM), and ligands stimulating cAMP-dependent protein kinase A (PKA) have been reported to enhance the activity of AR or its coactivators in CRPC .
The AF-1 region in the amino-terminal domain (NTD) of AR plays a primary role in AR-mediated gene expression in the castration-resistant stage . One of the mechanisms by which CRPC cells may evade androgen ablation is synthesis of splice variants of AR, which lack the ligand-binding domain, but retain AR NTD . In the absence of the ligand-binding domain at the AR C-terminus, the NTD drives constitutive transcriptional activity. Such splice variants lacking the ligand-binding domain have been detected in tissues isolated from patients with CRPC [55–57]. Recently, Andersen et al. have developed a small molecule, EPI-001, which can block transactivation of NTD and is reported to be specific for inhibition of AR without attenuating the transcriptional activities of related steroid receptors .
Modulation of Androgen–AR Axis in the Treatment of Prostate Cancer
As androgens stimulate the growth and survival of prostate cancer cells, the goal of hormonal therapy in prostate cancer is to deprive these cancer cells of androgen so that they die due to apoptosis [4, 5]. Therefore, regression of an androgen-dependent prostate tumor is induced either medically with luteinizing hormone-releasing hormone analogues or surgically by orchiectomy, which reduces the intracellular concentration of DHT . This in turn results in the death of androgen-sensitive cancer cells [4, 5]. Thus, androgen deprivation slows the progression of the tumor and enhances the quality of life. However, with time the disease inevitably recurs, and importantly, CRPC still remains dependent on the AR as discussed above .
Substantial levels of androgens have been measured in locally recurrent malignant prostate tumors and in intratumoral metastatic castration-recurrent prostate cancer tissues . These androgens in turn bind to AR and subsequently stimulate expression of androgen-regulated genes such as PSA, which commonly precedes clinical failure. Moreover, although the source of these tissue androgens may be either adrenal or intratumoral, targeting AR signaling in CRPC patients after androgen deprivation therapy by further blocking adrenal enzymes engaged in the biosynthesis of testosterone and DHT has been shown to be effective . Recently, abiraterone, a selective, steroidal irreversible inhibitor of CYP17 (17 hydroxylase/C17, 20-lyase), which blocks enzymes critical for the synthesis of testosterone, has been evaluated in patients with CRPC. Early results have shown promise with greater than a 50% decline in PSA levels in 67% of patients with advanced, CRPC, and objective radiologic responses in patients deriving no benefit from standard androgen ablation therapy . Another means of decreasing active androgen levels in target tissues is through inhibition of 5αR enzymes. Thus, finasteride, which primarily targets type 2 5αR, and dutasteride, which inhibits both 5αR isoenzymes, have been successfully used for the prevention of prostate cancer .
Androgens acting via AR play critical roles in the growth and survival of prostate cancer cells. However, there are distinct differences between the functions of androgens in normal and malignant prostate. Furthermore, although androgens promote differentiation in normal prostate, these hormones stimulate oncogenic potential in prostate cancer cells . As androgen plays a critical role in prostate carcinogenesis, the androgen signaling axis is an important therapeutic target in prostate cancer patients.
Conflict of Interest
The authors declare that they have no conflict of interest.