Date: 10 May 2011
Detection of Aflatoxin B1 with a Label-Free Ellipsometry Immunosensor
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The method of spectroscopic ellipsometry in total internal reflection mode (TIRE) was utilised for detection of Aflatoxin B1. The method of TIRE with the improved data analysis was capable of detection of aflatoxin molecules in low concentrations (down to 0.04 ng/ml) using a label-free and cost-effective direct immunoassay format. TIRE study of the binding kinetics yielded a large value of the association constant in the range of 106 l mol−1 which is typical for highly specific immune reactions. The comparison of the experimental data for three mycotoxins studied (e.g. aflatoxin B1, T-2 mycotoxin, and zearalenone) confirmed a common mechanism of the sensitivity boost due to the aggregation of hydrophobic molecules of mycotoxins in aqueous solutions.
Asoa, T., Buchi, G., Abdel-Kader, M. M., Chang, S. B., Wick, E. L., Wogan, G. N. (1963). Mycotoxins B and D. Journal of the American Chemical Society, 85, 1706–1707.CrossRef
Hudler, G. (1998). Magical mushrooms, mischievous mold. Princeton, NJ: Princeton University Press.
Peraica, M., Radic, B., Lucic, A., Pavlovic, M. (1999). Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization, 77, 754–766.
Cho, S. H., Lee, C. H., Jang, M. R., Son, J. W., Lee, S. M., Choi, I. S., et al. (2008). Aflatoxins contamination in spices and processed spice products commercialized in Korea. Food Chemistry, 107, 1283–1288.CrossRef
Saleemullah, I. A., Khalil, I. A., Shah, H. (2006). Aflatoxin contents of stored and artificially inoculated cereals and nuts. Food Chemistry, 98, 699–703.CrossRef
Kershaw, S. J. (1982). Occurrence of aflatoxins in oil seeds providing cocoa-butter substitutes. Environmental Microbiology, 43, 1210–1212.
Moss, M. (2002). Risk assessment for aflatoxins in foodstuffs. International Biodeterioration and Biodegradation, 50, 137–142.CrossRef
Wild, C. P., & Gong, Y. Y. (2010). Mycotoxins and human disease: A largely ignored global health issue. Carcinogenesis, 31, 71–82.CrossRef
Höhler, D. (2000). A brief survey on important mycotoxins and possible detoxification methods. Feed Tech, 4, 44–46.
European Commission. (1998). Commission Regulation (EC) No. 1525/98. J Eur Commun, L 201, 43–46.
Nabok, A., Tsargorodskaya, A., Hassan, A. K., Starodub, N. F. (2005). Total internal reflection ellipsometry and SPR detection of low molecular weight environmental toxins. Applied Surface Science, 246, 381–386.CrossRef
Nabok, A., & Tsargorodskaya, A. (2008). The method of total internal reflection ellipsometry for thin film characterisation and sensing. Thin Solid Films, 516, 8993–9000.CrossRef
Nabok, A., Tsargorodskaya, A., Holloway, A., Starodub, N. F., Gojster, O. (2007). Registration of T-2 Mycotoxin with total internal reflection ellipsometry and QCM impedance methods. Biosensors & Bioelectronics, 22, 885–890.CrossRef
Nabok, A., Tsargorodskaya, A., Holloway, A., Starodub, N. F., Demchenko, A. (2007). Specific binding of large aggregates of amphiphilic molecules to respective antibodies. Langmuir, 23, 8485–8490.CrossRef
Westpal, P., & Bornmann, A. (2002). Biomolecular detection by surface plasmon enhanced ellipsometry. Sensors & Actuators B, 84, 278–282.CrossRef
Arwin, H., Poksinski, M., Johansen, K. (2004). Total internal reflection ellipsometry: Principles and applications. Appl Optics, 43, 3028–3036.CrossRef
Nabok, A., Tsargorodskaya, A., Mustafa, M.K., Szekacs, I., Starodub, N.F., Szekacs, A. (2011). Detection of low molecular weight toxins using optical phase detection techniques. Sensors Actuators B: Chemical, 211, 232–237.
Lvov, Y., & Decher, G. (1994). Assembly of multilayer ordered films by alternating adsorption of oppositely charged macromolecules. Crystallography Reports, 39, 616–696.
Lvov Y. & Mohwald, H. (Eds.) (2000). Protein Architecture. Interfacing Molecular Assemblies and Immobilization Biotechnology, Marcel Dekker.
Nabok, A. (2005). Organic and inorganic nano-structures. Boston: Artech.
Suryajaya, N. A. V., Tsargorodskaya, A., Hassan, A. K., Davis, F. (2008). Electrostatically self-assembled films containing II–VI semiconductor nanoparticles: Optical and electrical properties. Thin Solid Films, 516, 8917–8925.CrossRef
Woollam JA (2002). Guide to using WVASE32, J. A. Woollam
Székács, A., Adányi, N., Székács, I., Majer-Baranyi, K., Szendrő, I. (2009). Optical waveguide lightmode spectroscopy immunosensors for environmental monitoring. Appl Optics, 48, B151–B158.CrossRef
Lishchuk, S., Tsargorodskaya, A., Nabok, A. (2008). The model of alkylphenol micelles bound to respective antibodies on the solid surface. Colloids and Surfaces. A, 324, 117–121.CrossRef
Liu, X., Wei, J., Song, D., Zhang, Z., Zhang, H., Luo, G. (2003). Determination of affinities and antigenic epitopes of bovine cardiac troponin I (cTnI) with monoclonal antibodies by surface plasmon resonance biosensor. Analytical Biochemistry, 314, 301–309.CrossRef
- Detection of Aflatoxin B1 with a Label-Free Ellipsometry Immunosensor
Volume 1, Issue 1-2 , pp 38-45
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- Total internal reflection ellipsometry
- Direct immunoassay
- Immune reaction kinetic
- Aggregation of mycotoxins
- Industry Sectors
- Author Affiliations
- 1. Materials and Engineering Research Institute, Sheffield Hallam University, Sheffield, S1 1WB, UK
- 2. National University of Life and Environmental Sciences of Ukraine, Kiev, 03041, Ukraine