Neurotoxicity Research

, Volume 17, Issue 4, pp 435–439

Caffeine Enhances Astroglia and Microglia Reactivity Induced by 3,4-Methylenedioxymethamphetamine (‘Ecstasy’) in Mouse Brain

  • Amit Khairnar
  • Antonio Plumitallo
  • Lucia Frau
  • Nicoletta Schintu
  • Micaela Morelli
Article

DOI: 10.1007/s12640-009-9125-y

Cite this article as:
Khairnar, A., Plumitallo, A., Frau, L. et al. Neurotox Res (2010) 17: 435. doi:10.1007/s12640-009-9125-y

Abstract

Several reports suggest that 3,4-methylenedioxymethamphetamine (MDMA) induces neurotoxic effects and gliosis. Since recreational use of MDMA is often associated with caffeinated beverages, we investigated whether caffeine interferes with MDMA-induced astroglia and microglia activation, thus facilitating its neurotoxicity. MDMA (4 × 20 mg/kg) was acutely administered to mice alone or in combination with caffeine (10 mg/kg). CD11b and GFAP immunoreactivity were evaluated as markers of microglia and astroglia activation in the substantia nigra pars-compacta (SNc) and striatum. MDMA was associated with significantly higher CD11b and GFAP immunoreactivity in striatum, whereas only CD11b was significantly higher than vehicle in SNc. Caffeine potentiated the increase in CD11b and GFAP in the striatum but not in the SNc of MDMA-treated mice. The abuse of MDMA is a growing worldwide problem; the results of this study suggest that combination of MDMA plus caffeine by increasing glial activation might have harmful health consequences.

Keywords

Glial cell Adenosine Drug of abuse Striatum Psychostimulants 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Amit Khairnar
    • 1
  • Antonio Plumitallo
    • 2
  • Lucia Frau
    • 1
  • Nicoletta Schintu
    • 1
  • Micaela Morelli
    • 1
    • 3
    • 4
  1. 1.Department of ToxicologyUniversity of CagliariCagliariItaly
  2. 2.Department of Pharmaceutical Chemistry and TechnologyUniversity of CagliariCagliariItaly
  3. 3.Centre of Excellence for Neurobiology of DependenceUniversity of CagliariCagliariItaly
  4. 4.CNR Institute of NeuroscienceUniversity of CagliariCagliariItaly