, Volume 16, Issue 1, pp 30-41

The Janus Face of Resveratrol in Astroglial Cells

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Abstract

Astroglial cells are key modulators of neuropathology events. Resveratrol, a redox-active compound present in grapes and wine, has a wide range of biological effects. The aim of this study was to investigate whether resveratrol is able to prevent hydrogen peroxide (H2O2)-induced oxidative damage in C6 astroglial cells. We found that following a short oxidative insult (Model I—1 mM H2O2/30 min), resveratrol increased glutamate uptake (60%), glutamine synthetase (GS) (139%), glutathione (GSH) (120%), and S100B secretion (24%); and attenuated DCFH oxidation (34%) as compared to H2O2 values. Under less intense (0.1 mM H2O2), but lasting (6 h) insult (Model II), resveratrol had an opposite effect, potentiating the H2O2-induced decrease in glutamate uptake (from 34 to 63%), in GS (from 22 to 50%), in GSH (from 22 to 54%), and also potentiating DCFH oxidation (from 24 to 38%). The transcription factor, NF-κB, was activated in both models. Cell morphology alterations were also observed in the presence of H2O2 with process-bearing cells, accompanied by cell body retraction and actin reorganization. This effect was not prevented by resveratrol, but was prevented by lysophosphatidic acid (LPA), a specific upstream positive regulator of Rho A. In summary, these findings showed that resveratrol, a redox-active compound, was able to modulate important neurotrophic function of astroglial cells under different oxidative conditions.