Effect of an 8-weeks aerobic training program in elderly on oxidative stress and Hsp72 expression in leukocytes during antioxidant supplementation
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To investigate the effect of aerobic training in the context of antioxidant supplementation on systemic oxidative stress and leukocytes heat shock protein (Hsp)72 expression in the elderly.
Sixteen septuagenarians (8 males and 8 females, mean age 74.6) were supplemented with Vitamin C and E (respectively 500 and 100mg per day) and randomly assigned either to sedentary (AS) or individualized aerobically trained (AT) group for 8 weeks.
Plasma Vitamin C and E concentrations and aerobic fitness, as well as resting and post graded exercise (GXT) Hsp72 expression in leukocytes, plasma levels of thiobarbituric acid reactive substances (TBARS) and advanced oxidation protein product (AOPP) were measured pre and post training / supplementation.
At the end of the intervention, the two groups showed a significant increase in resting plasma vitamin C and E (approximately 50 and 20% increase respectively) and a significant decrease in both resting and post GXT plasma TBARS and AOPP (approximately 25 and 20% decrease respectively). These changes were of similar magnitude in the two groups. The reduced oxidative stress was concomitant with a 15% decreased expression of Hsp72 in monocytes and granulocytes in both groups.
This study provides evidence that in elderly, increased concentration of antioxidant vitamins C and E is associated with a reduction in oxidative stress and leukocytes Hsp72. In this context, 8 weeks of aerobic training has no impact on oxidative stress or leukocytes Hsp72 expression in elderly people.
- Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1956; 11: 298–300.
- Sierra F. Is (your cellular response to) stress killing you? J Gerontol A Biol Sci Med Sci. 2006; 61: 557–561. CrossRef
- Ji LL, Leeuwenburgh C, Leichtweis S, Gore M, Fiebig R, Hollander J, and Bejma J. Oxidative stress and aging. Role of exercise and its influences on antioxidant systems. Ann N Y Acad Sci. 1998; 854: 102–117. CrossRef
- Thomas PJ, Qu BH, and Pedersen PL. Defective protein folding as a basis of human disease. Trends Biochem Sci. 1995; 20: 456–459. CrossRef
- Hutter JJ, Mestril R, Tam EK, Sievers RE, Dillmann WH, and Wolfe CL. Overexpression of heat shock protein 72 in transgenic mice decreases infarct size in vivo. Circulation. 1996; 94: 1408–1411.
- McArdle A, Dillmann WH, Mestril R, Faulkner JA, and Jackson MJ. Overexpression of HSP70 in mouse skeletal muscle protects against muscle damage and age-related muscle dysfunction. Faseb J. 2004; 18: 355–357.
- Powers SK, Demirel HA, Vincent HK, Coombes JS, Naito H, Hamilton KL, Shanely RA, and Jessup J. Exercise training improves myocardial tolerance to in vivo ischemia-reperfusion in the rat. Am J Physiol. 1998; 275: R1468–1477.
- Locke M and Tanguay RM. Diminished heat shock response in the aged myocardium. Cell Stress Chaperones. 1996; 1: 251–260. CrossRef
- Rao DV, Watson K, and Jones GL. Age-related attenuation in the expression of the major heat shock proteins in human peripheral lymphocytes. Mech Ageing Dev. 1999; 107: 105–118. CrossRef
- Radak Z, Chung HY, and Goto S. Exercise and hormesis: oxidative stress-related adaptation for successful aging. Biogerontology. 2005; 6: 71–75. CrossRef
- Clarkson PM and Thompson HS. Antioxidants: what role do they play in physical activity and health? Am J Clin Nutr. 2000; 72: 637S–646S.
- Jessup JV, Horne C, Yarandi H, and Quindry J. The effects of endurance exercise and vitamin E on oxidative stress in the elderly. Biol Res Nurs. 2003; 5: 47–55. CrossRef
- Ruiz-Ramos M, Vargas LA, Fortoul Van der Goes TI, Cervantes-Sandoval A, and Mendoza-Nunez VM. Supplementation of ascorbic acid and alpha-tocopherol is useful to preventing bone loss linked to oxidative stress in elderly. J Nutr Health Aging. 2010; 14: 467–472. CrossRef
- Khassaf M, McArdle A, Esanu C, Vasilaki A, McArdle F, Griffiths RD, Brodie DA, and Jackson MJ. Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol. 2003; 549: 645–652. CrossRef
- Peng J, Jones GL, and Watson K. Stress proteins as biomarkers of oxidative stress: effects of antioxidant supplements. Free Radic Biol Med. 2000; 28: 1598–1606. CrossRef
- Fehrenbach E, Passek F, Niess AM, Pohla H, Weinstock C, Dickhuth HH, and Northoff H. HSP expression in human leukocytes is modulated by endurance exercise. Med Sci Sports Exerc. 2000; 32: 592–600. CrossRef
- Hamilton KL, Staib JL, Phillips T, Hess A, Lennon SL, and Powers SK. Exercise, antioxidants, and HSP72: protection against myocardial ischemia/reperfusion. Free Radic Biol Med. 2003; 34: 800–809. CrossRef
- Liu Y, Lormes W, Baur C, Opitz-Gress A, Altenburg D, Lehmann M, and Steinacker JM. Human skeletal muscle HSP70 response to physical training depends on exercise intensity. Int J Sports Med. 2000; 21: 351–355. CrossRef
- Simar D, Malatesta D, Badiou S, Dupuy AM, and Caillaud C. Physical activity modulates heat shock protein-72 expression and limits oxidative damage accumulation in a healthy elderly population aged 60 90 years. J Gerontol A Biol Sci Med Sci. 2007; 62: 1413–1419. CrossRef
- Margaritis I and Rousseau AS. Does physical exercise modify antioxidant requirements? Nutr Res Rev. 2008; 21: 3–12. CrossRef
- Voorrips LE, Ravelli AC, Dongelmans PC, Deurenberg P, and Van Staveren WA. A physical activity questionnaire for the elderly. Med Sci Sports Exerc. 1991; 23: 974–979.
- Simar D, Malatesta D, Koechlin C, Cristol JP, Vendrell JP, and Caillaud C. Effect of age on Hsp72 expression in leukocytes of healthy active people. Exp Gerontol. 2004; 39: 1467–1474. CrossRef
- Malatesta D, Simar D, Dauvilliers Y, Candau R, Borrani F, Prefaut C, and Caillaud C. Energy cost of walking and gait instability in healthy 65- and 80-yr-olds. J Appl Physiol. 2003; 95: 2248–2256.
- Salonen RM, Nyyssonen K, Kaikkonen J, Porkkala-Sarataho E, Voutilainen S, Rissanen TH, Tuomainen TP, Valkonen VP, Ristonmaa U, Lakka HM, Vanharanta M, Salonen JT, and Poulsen HE. Six-year effect of combined vitamin C and E supplementation on atherosclerotic progression: the Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) Study. Circulation. 2003; 107: 947–953. CrossRef
- Ahmaidi S, Masse-Biron J, Adam B, Choquet D, Freville M, Libert JP, and Prefaut C. Effects of interval training at the ventilatory threshold on clinical and cardiorespiratory responses in elderly humans. Eur J Appl Physiol Occup Physiol. 1998; 78: 170–176. CrossRef
- Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 1976; 15: 212–216. CrossRef
- Witko-Sarsat V, Friedlander M, Nguyen Khoa T, Capeillere-Blandin C, Nguyen AT, Canteloup S, Dayer JM, Jungers P, Drueke T, and Descamps-Latscha B. Advanced oxidation protein products as novel mediators of inflammation and monocyte activation in chronic renal failure. J Immunol. 1998; 161: 2524–2532.
- Cachia O, Leger CL, Boulot P, Vernet MH, Michel F, Crastes de Paulet A, and Descomps B. Red blood cell vitamin E concentrations in fetuses are related to but lower than those in mothers during gestation. A possible association with maternal lipoprotein (a) plasma levels. Am J Obstet Gynecol. 1995; 173: 42–51. CrossRef
- Washko PW, Welch RW, Dhariwal KR, Wang Y, and Levine M. Ascorbic acid and dehydroascorbic acid analyses in biological samples. Anal Biochem. 1992; 204: 1–14. CrossRef
- Godel JC. Vitamin E status of northern Canadian newborns: relation of vitamin E to blood lipids. Am J Clin Nutr. 1989; 50: 375–380.
- Gaskill SE, Walker AJ, Serfass RA, Bouchard C, Gagnon J, Rao DC, Skinner JS, Wilmore JH, and Leon AS. Changes in ventilatory threshold with exercise training in a sedentary population: the HERITAGE Family Study. Int J Sports Med. 2001; 22: 586–592. CrossRef
- Malatesta D, Simar D, Dauvilliers Y, Candau R, Ben Saad H, Prefaut C, and Caillaud C. Aerobic determinants of the decline in preferred walking speed in healthy, active 65- and 80-year-olds. Pflugers Arch. 2004; 447: 915–921. CrossRef
- Belman MJ and Gaesser GA. Exercise training below and above the lactate threshold in the elderly. Med Sci Sports Exerc. 1991; 23: 562–568.
- Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, and Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000; 32: 1576–1581. CrossRef
- Koechlin C, Couillard A, Simar D, Cristol JP, Bellet H, Hayot M, and Prefaut C. Does oxidative stress alter quadriceps endurance in chronic obstructive pulmonary disease? American Journal of Respiratory and Critical Care Medicine. 2004; 169: 1022–1027. CrossRef
- Buttke TM and Sandstrom PA. Oxidative stress as a mediator of apoptosis. Immunol Today. 1994; 15: 7–10. CrossRef
- Nelson ME, Rejeski WJ, Blair SN, Duncan PW, Judge JO, King AC, Macera CA, and Castaneda-Sceppa C. Physical activity and public health in older adults: recommendation from the American College of Sports Medicine and the American Heart Association. Circulation. 2007; 116: 1094–1105. CrossRef
- Niess AM, Fehrenbach E, Schlotz E, Sommer M, Angres C, Tschositsch K, Battenfeld N, Golly IC, Biesalski HK, Northoff H, and Dickhuth HH. Effects of RRR-alpha-tocopherol on leukocyte expression of HSP72 in response to exhaustive treadmill exercise. Int J Sports Med. 2002; 23: 445–452. CrossRef
- Fehrenbach E, Niess AM, Veith R, Dickhuth HH, and Northoff H. Changes of HSP72-expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature. J Leukoc Biol. 2001; 69: 747–754.
- Miller AA, De Silva TM, Jackman KA, and Sobey CG. Effect of gender and sex hormones on vascular oxidative stress. Clin Exp Pharmacol Physiol. 2007; 34: 1037–1043. CrossRef
- Hertogh EM, Monninkhof EM, Schouten EG, Peeters PH, and Schuit AJ. Validity of the Modified Baecke Questionnaire: comparison with energy expenditure according to the doubly labeled water method. Int J Behav Nutr Phys Act. 2008; 5: 30. CrossRef
- Effect of an 8-weeks aerobic training program in elderly on oxidative stress and Hsp72 expression in leukocytes during antioxidant supplementation
The journal of nutrition, health & aging
Volume 16, Issue 2 , pp 155-161
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- Oxidative damage
- Heat shock proteins
- Physical activity
- Industry Sectors
- Author Affiliations
- 1. School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- 6. School of Medical Science, Faculty of Medicine, University of New South Wales, Wallace Wurth Building, Sydney, NSW, 2052, Australia
- 2. INSERM, ERI 25 “Muscle and Pathologies”, Hôpital A. de Villeneuve, 34295, Montpellier, France
- 5. Institute of Sport Sciences (ISSUL), Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland
- 3. Département de Biochimie, Hôpital Lapeyronie, 34295, Montpellier, France
- 4. Exercise, Health & Performance Research Group, Faculty of Health Sciences, The University of Sydney, Sydney, Australia