Probiotics and Antimicrobial Proteins

, Volume 5, Issue 3, pp 216–226

Toward an Alternative Therapeutic Approach for Skin Infections: Antagonistic Activity of Lactobacilli Against Antibiotic-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

  • Mohamed M. Hafez
  • Ibrahim A. Maghrabi
  • Noha M. Zaki
Article

DOI: 10.1007/s12602-013-9137-z

Cite this article as:
Hafez, M.M., Maghrabi, I.A. & Zaki, N.M. Probiotics & Antimicro. Prot. (2013) 5: 216. doi:10.1007/s12602-013-9137-z

Abstract

The wide spread of antimicrobial resistance has urged the need of alternative therapeutic approach. In this context, probiotic lactobacilli have been reported for the prevention and treatment of many gastrointestinal and urogenital infections. However, very little is known about their antagonistic activity against skin pathogens. Accordingly, the present study aimed to investigate the potential of lactobacilli to interfere with pathogenesis features of two antibiotic-resistant skin pathogens, namely methicillin-resistant Staphylococcus aureus and multiple-resistant Pseudomonas aeruginosa. A total of 49 lactobacilli were recovered, identified and tested for their antagonistic activities against the aforementioned pathogens. Of these, eight isolates were capable of blocking the adherence of pathogens to mammalian cells independent of the skin pathogen tested or model adopted. Moreover, three Lactobacillus isolates (LRA4, LC2 and LR5) effectively prevented the pathogen internalization into epithelial cells in addition to potentiating phagocyte-mediated pathogen killing. Interestingly, the lactobacilli LC2, LF9 and LRA4 markedly inhibited the growth of P. aeruginosa and S. aureus isolates in coculture experiments. Besides, the lactobacilli LRA4, LC2, LR5 and LF9 have counteracted pathogen cytotoxicity. Taken together, the present study revealed some inhibitory activities of lactobacilli against two antibiotic-resistant skin pathogens. Moreover, it revealed two lactobacilli, namely LC2 and LRA4, with antagonistic capacity against different virulence determinants of skin pathogens. These lactobacilli are considered promising probiotic candidates that may represent an alternative therapeutic approach for skin infections.

Keywords

Skin infectionPseudomonas aeruginosaStaphylococcus aureusAdherenceInternalizationCytotoxicity

Supplementary material

12602_2013_9137_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 22 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mohamed M. Hafez
    • 1
  • Ibrahim A. Maghrabi
    • 2
  • Noha M. Zaki
    • 3
  1. 1.Department of Pharmaceutical Microbiology, College of PharmacyTaif UniversityTaif, Western RegionKSA
  2. 2.Department of Clinical Pharmacy, College of PharmacyTaif UniversityTaifKSA
  3. 3.Department of Pharmaceutics, College of PharmacyTaif UniversityTaifKSA