Abstract
The diversity of begomovirus and associated betasatellite complexes was analyzed from infected leaf samples of radish, tomato, chili, torai (ridge gourd), cotton, spinach, citrus and guar bean collected from different geographical regions of northern India. Leaves showing the characteristic begomovirus symptoms were used for cloning and sequencing for further characterization of the begomovirus complexes. In the present study, coat protein (CP) was amplified from eight different infected crop samples and betasatellites from only six. Our results showed significant diversity of CP and betasatellites of monopartite begomovirus in different crops in northern India. Phylogenetic analysis of CP and betasatellites test sequences exhibit a close relationship to diverse crops infecting begomovirus complexes. This strengthens the increase of host range of begomovirus in India.
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The authors are thankful to Department of Science and Technology DST project no. SR/FT/LS-042/2009), and the Council of Scientific & Industrial Research, India, for financial support.
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Anurag Kumar Sahu and Chitra Nehra contributed equally to the research.
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Sahu, A.K., Nehra, C. & Gaur, R.K. Molecular diversity of monopartite begomovirus coat protein and betasatellite associated with different crop species in India. Phytoparasitica 43, 81–85 (2015). https://doi.org/10.1007/s12600-014-0418-1
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DOI: https://doi.org/10.1007/s12600-014-0418-1