The Journal of Physiological Sciences

, Volume 59, Issue 2, pp 113–122

Involvement of aquaporin-5 in differentiation of human gastric cancer cells

  • Tomoko Watanabe
  • Takuto Fujii
  • Takeshi Oya
  • Naoki Horikawa
  • Yoshiaki Tabuchi
  • Yuji Takahashi
  • Magotoshi Morii
  • Noriaki Takeguchi
  • Kazuhiro Tsukada
  • Hideki Sakai
Original Paper

DOI: 10.1007/s12576-008-0017-3

Cite this article as:
Watanabe, T., Fujii, T., Oya, T. et al. J Physiol Sci (2009) 59: 113. doi:10.1007/s12576-008-0017-3
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Abstract

Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl2, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.

Keywords

Aquaporins Stomach Cancer cells 

Copyright information

© The Physiological Society of Japan and Springer 2009

Authors and Affiliations

  • Tomoko Watanabe
    • 1
  • Takuto Fujii
    • 2
  • Takeshi Oya
    • 3
  • Naoki Horikawa
    • 1
  • Yoshiaki Tabuchi
    • 4
  • Yuji Takahashi
    • 2
  • Magotoshi Morii
    • 5
  • Noriaki Takeguchi
    • 2
  • Kazuhiro Tsukada
    • 1
  • Hideki Sakai
    • 2
  1. 1.Department of Surgery II, Graduate School of Medicine and Pharmaceutical SciencesUniversity of ToyamaToyamaJapan
  2. 2.Department of Pharmaceutical Physiology, Graduate School of Medicine and Pharmaceutical SciencesUniversity of ToyamaToyamaJapan
  3. 3.Department of Pathology II, Graduate School of Medicine and Pharmaceutical SciencesUniversity of ToyamaToyamaJapan
  4. 4.Life Science Research CenterUniversity of ToyamaToyamaJapan
  5. 5.Laboratory of Physical Chemistry, Faculty of Pharmaceutical SciencesSuzuka University of Medical ScienceMieJapan

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