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Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

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Abstract

An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrial-encoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1–8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.

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Acknowledgments

This work was supported by the Malaysian Government through the Ministry of Education under KPM-JSPS COMSEA Matching Fund (GA002-2014), the Higher Institution Centre of Excellence Research Grant (IOES-2014C); and the University of Malaya Research Program (RU006A/B-2014). This work formed part of the MSc project of SN Tan.

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Authors and Affiliations

  1. Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, 16310, Bachok, Kelantan, Malaysia

    Suh Nih Tan, Po Teen Lim & Chui Pin Leaw

  2. Tunku Abdul Rahman University College, Johor Branch Campus, 85000, Segamat, Johor, Malaysia

    Hong Chang Lim

  3. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia

    Sing Tung Teng

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  1. Suh Nih Tan
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Correspondence to Chui Pin Leaw.

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Tan, S.N., Lim, H.C., Teng, S.T. et al. Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fish Sci 81, 831–838 (2015). https://doi.org/10.1007/s12562-015-0902-7

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