Original Article Aquaculture

Fisheries Science

, Volume 79, Issue 1, pp 119-127

Toxicity and absorption of dietary leucomalachite green in Nile tilapia Oreochromis niloticus

  • Mintra Seel-audomAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology
  • , Laddawan KrongpongAffiliated withDepartment of Fisheries, Inland Feed Research Institute
  • , Kunihiko FutamiAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology Email author 
  • , Ana Teresa GonçalvesAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology
  • , Takayuki KatagiriAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology
  • , Nontawith AreechonAffiliated withDepartment of Aquaculture, Faculty of Fisheries, Kasetsart University
  • , Makoto EndoAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology
  • , Masashi MaitaAffiliated withGraduate School of Marine Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology

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Abstract

Farmed fish are exposed to risks from feed-borne chemical contamination, such as leucomalachite green (LMG) in fish meal. Consequently, the use of malachite green is prohibited in aquacultural practice. An improved understanding of the toxicity of dietary LMG provided to farmed fish is needed in order to manage risk. Oreochromis niloticus specimens were fed experimental diets containing 0, 100, 500, and 2500 μg/kg LMG for 28 days. On sampling days 7, 14, and 28, fish in the exposed groups had detectable levels of LMG. Accumulation levels were approximately 12.2 % (in the liver) and 6.2 % (in the muscle) of the LMG concentration in the feed (104, 510, and 2200 μg/kg). Hematocrit and mean corpuscular hemoglobin concentrations in the 500 and 2500 μg/kg groups were significantly elevated at 7 and 14 days. Hemoglobin in the group that received the highest dose was significantly higher than that in the control group. Significant increases in the activities of alanine aminotransferase and alkaline phosphatase were also detected in the group receiving the highest dose. Total cholesterol concentrations in all of the exposed groups were significantly higher than those in the control group. These observations of toxicity were dose dependent. Histological changes in gills and livers were observed in LMG-exposed fish.

Keywords

Nile tilapia Leucomalachite green Dietary exposure Toxicity