Original Article Food Science and Technology

Fisheries Science

, Volume 78, Issue 1, pp 197-206

First online:

Species identification method for marine products of Seriola and related species

  • Jun IguchiAffiliated withFood and Agricultural Materials Inspection Center Email author 
  • , Yasuharu TakashimaAffiliated withFood and Agricultural Materials Inspection Center
  • , Atsushi NamikoshiAffiliated withFood and Agricultural Materials Inspection Center, Kobe Regional Center
  • , Michiaki YamashitaAffiliated withNational Research Institute of Fisheries Science, Fisheries Research Agency

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The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S. rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphe japonica, and Rachycentron canadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a 373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species.


Hyperoglyphe japonica PCR–RFLP Rachycentron canadum Seriola spp. Seriolella spp. Species identification