Fisheries Science

, Volume 75, Issue 3, pp 771–776

Direct contact between Pseudo-nitzschia multiseries and bacteria is necessary for the diatom to produce a high level of domoic acid

  • Kenji Kobayashi
  • Yoshinobu Takata
  • Masaaki Kodama
Original Article Chemistry and Biochemistry

DOI: 10.1007/s12562-009-0081-5

Cite this article as:
Kobayashi, K., Takata, Y. & Kodama, M. Fish Sci (2009) 75: 771. doi:10.1007/s12562-009-0081-5

Abstract

Pseudo-nitzschia multiseries loses most of its ability to produce domoic acid when it is cultured axenically. However, domoic acid production recovers when bacteria from the original culture are reintroduced to axenic cultures, indicating a bacterial association with domoic acid production in this species. In order to determine the role of bacteria in domoic acid production, an axenic strain prepared from a nonaxenic strain of P. multiseries was inoculated into media in cellophane tubes, which were then placed in a bottle containing the original nonaxenic culture. Both strains showed characteristic domoic acid production similar to that previously observed. Domoic acid level in the cells of the axenic strain in the tubes was much smaller than that of the cells outside the tubes. These results show that direct contact between living bacteria and P. multiseries is necessary for producing high levels of domoic acid in P. multiseries.

Keywords

Co-culture of axenic and nonaxenic strainsDomoic acidPseudo-nitzschia multiseries

Copyright information

© The Japanese Society of Fisheries Science 2009

Authors and Affiliations

  • Kenji Kobayashi
    • 1
  • Yoshinobu Takata
    • 1
  • Masaaki Kodama
    • 1
  1. 1.School of Marine BiosciencesKitasato UniversityOfunatoJapan