Original Paper

Food and Environmental Virology

, Volume 4, Issue 2, pp 68-72

First online:

Comparative Analysis of Viral Concentration Methods for Detecting the HAV Genome Using Real-Time RT-PCR Amplification

  • Kang Bum LeeAffiliated withDepartment of Food and Nutrition, School of Food Science and Technology, Chung-Ang University
  • , Hyeokjin LeeAffiliated withDivision of Hepatitis Virus, Korea Center for Disease Control and Prevention
  • , Sang-Do HaAffiliated withSchool of Food Science and Technology, Chung-Ang University
  • , Doo-Sung CheonAffiliated withDivision of Hepatitis Virus, Korea Center for Disease Control and Prevention Email author 
  • , Changsun ChoiAffiliated withDepartment of Food and Nutrition, School of Food Science and Technology, Chung-Ang UniversitySchool of Food Science and Technology, Chung-Ang University Email author 

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Abstract

Hepatitis A is a major infectious disease epidemiologically associated with foodborne and waterborne outbreaks. Molecular detection using real-time RT-PCR to detect the hepatitis A virus (HAV) in contaminated vegetables can be hindered by low-virus recoveries during the concentration process and by natural PCR inhibitors in vegetables. This study evaluated three virus concentration methods from vegetables: polyethylene glycol (PEG) precipitation, ultrafiltration (UF), and immunomagnetic separation (IMS). UF was the most efficient concentration method, while PEG and IMS were very low for the recovery rate of HAV. These results demonstrate that UF is the most appropriate method for recovering HAV from contaminated vegetables and that this method combined with the real-time RT-PCR assay may be suitable for routine laboratory use.

Keywords

Hepatitis A virus (HAV) Concentration Detection Ultrafiltration (UF) Immunomagnetic separation (IMS)