Food and Environmental Virology

, Volume 4, Issue 2, pp 68–72

Comparative Analysis of Viral Concentration Methods for Detecting the HAV Genome Using Real-Time RT-PCR Amplification

Original Paper

DOI: 10.1007/s12560-012-9077-x

Cite this article as:
Lee, K.B., Lee, H., Ha, SD. et al. Food Environ Virol (2012) 4: 68. doi:10.1007/s12560-012-9077-x


Hepatitis A is a major infectious disease epidemiologically associated with foodborne and waterborne outbreaks. Molecular detection using real-time RT-PCR to detect the hepatitis A virus (HAV) in contaminated vegetables can be hindered by low-virus recoveries during the concentration process and by natural PCR inhibitors in vegetables. This study evaluated three virus concentration methods from vegetables: polyethylene glycol (PEG) precipitation, ultrafiltration (UF), and immunomagnetic separation (IMS). UF was the most efficient concentration method, while PEG and IMS were very low for the recovery rate of HAV. These results demonstrate that UF is the most appropriate method for recovering HAV from contaminated vegetables and that this method combined with the real-time RT-PCR assay may be suitable for routine laboratory use.


Hepatitis A virus (HAV)ConcentrationDetectionUltrafiltration (UF)Immunomagnetic separation (IMS)

Copyright information

© Springer Science + Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Food and Nutrition, School of Food Science and TechnologyChung-Ang UniversityAnsung-siSouth Korea
  2. 2.Division of Hepatitis VirusKorea Center for Disease Control and PreventionCheongwonSouth Korea
  3. 3.School of Food Science and TechnologyChung-Ang UniversityAnsung-siSouth Korea