, Volume 1, Issue 1, pp 37-42,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 17 Jan 2009

Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures

Abstract

After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field or magnetic field is also addressed.