Mycotoxin Research

, Volume 31, Issue 2, pp 109–115

A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples

  • Sebastian Hickert
  • Johannes Gerding
  • Edson Ncube
  • Florian Hübner
  • Bradley Flett
  • Benedikt Cramer
  • Hans-Ulrich Humpf
Original Paper

DOI: 10.1007/s12550-015-0221-y

Cite this article as:
Hickert, S., Gerding, J., Ncube, E. et al. Mycotoxin Res (2015) 31: 109. doi:10.1007/s12550-015-0221-y

Abstract

Using micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) a simple and fast method for the quantitative determination of 26 mycotoxins was developed. Sample preparation consists of a single extraction step and a dilute-and-shoot approach without further cleanup. With a total run time of 9 min and solvent consumption below 0.3 mL per chromatographic run, the presented method is cost-effective. All toxins regulated by the European Commission with maximum or guidance levels in grain products (fumonisins B1 and B2 (FB1 and FB2)); deoxynivalenol (DON); aflatoxins B1, G1, B2, and G2 (AFB1, AFG1, AFB2, and AFG2); ochratoxin A (OTA); T-2 and HT-2 toxins; and zearalenone (ZEN) can be quantified with this method. Furthermore, the enniatins B, B1, A, and A1 (EnB, EnB1, EnA, and EnA1); beauvericin (BEA); 3-acetyl-deoxynivalenol (3-AcDON); fusarin C (FusC); sterigmatocystin (STC); gliotoxin (GT); and the Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), tentoxin (TEN), and altertoxin I (ATX I) can also be quantified. For all regulated compounds, recoveries ranged between 76 and 120 %. For all other toxins, the recovery was at least 51 %. The method was applied for the analysis of 42 maize samples from field trials in South Africa.

Keywords

Micro LC Mass spectrometry Capillary chromatography Aflatoxin Multi-method 

Supplementary material

12550_2015_221_MOESM1_ESM.pdf (2.2 mb)
ESM 1(PDF 2211 kb)

Copyright information

© Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sebastian Hickert
    • 1
    • 2
  • Johannes Gerding
    • 1
    • 2
  • Edson Ncube
    • 3
  • Florian Hübner
    • 1
  • Bradley Flett
    • 3
    • 4
  • Benedikt Cramer
    • 1
  • Hans-Ulrich Humpf
    • 1
    • 2
  1. 1.Institute of Food ChemistryUniversity of MünsterMünsterGermany
  2. 2.NRW Graduate School of ChemistryMünsterGermany
  3. 3.ARC-Grain Crops InstitutePotchefstroomSouth Africa
  4. 4.Unit of Environmental Sciences and ManagementNorth-West UniversityPotchefstroomSouth Africa