Reproductive Medicine and Biology

, Volume 12, Issue 4, pp 167–171

Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes


  • Hidemi Yokota
    • Yokota Maternity Hospital
    • Yokota Maternity Hospital
  • Mikako Yokota
    • Yokota Maternity Hospital
  • Yasuyuki Araki
    • The Institute for Advanced Reproductive Medical Technology
  • Yasuhisa Araki
    • The Institute for Advanced Reproductive Medical Technology
Original Article

DOI: 10.1007/s12522-013-0150-4

Cite this article as:
Yokota, H., Yokota, Y., Yokota, M. et al. Reprod Med Biol (2013) 12: 167. doi:10.1007/s12522-013-0150-4



To evaluate the effect of long-term caffeine administration to mice on in vitro fertilization (IVF) of oocytes.


Mice were injected with different dosages (0, 0.1, and 1.0 mg/mouse/converted day) of caffeine for one month. Subsequently, the fertilization rate and embryo development to blastocyst stage were evaluated in IVF using oocytes from the mice.


The retrieved average oocyte rate was significantly lower (27.4) in mice injected with 1.0 mg caffeine than in the control group (36.5; P < 0.05); the fertilization rate was significantly different between the 0 mg (317/401; 79.1 %) and 1.0 mg group (199/301; 66.1 %) (P < 0.05). At 96 h after insemination, the blastocyst formation rate was significantly decreased in the 1.0 mg group (94/199; 47.2 %) compared with the control (0 mg) group (237/317; 74.8 %) and 0.1 mg group (226/323; 70 %) (P < 0.05). When 1.0 mg caffeine was administered for two weeks, embryo development was significantly impacted.


Our findings suggest that caffeine administration negatively impacts oocytogenesis and embryonic development after IVF.


ARTBlastocyst formationCaffeineHatchingIn vitro fertilization

Copyright information

© Japan Society for Reproductive Medicine 2013