Reproductive Medicine and Biology

, Volume 9, Issue 4, pp 197–203

Cryopreservation of ovarian tissue after pretreatment with a gonadotropin-releasing hormone agonist

Authors

  • Suguru Igarashi
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
  • Marie Osada
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
  • Seido Takae
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
  • Wataru Tarumi
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
  • Bunpei Ishizuka
    • Department of Obstetrics and GynecologySt. Marianna University School of Medicine
Original Article

DOI: 10.1007/s12522-010-0058-1

Cite this article as:
Igarashi, S., Suzuki, N., Osada, M. et al. Reprod Med Biol (2010) 9: 197. doi:10.1007/s12522-010-0058-1

Abstract

Purpose

The purpose of this study was to investigate whether slow-rate freezing or vitrification is better for cryopreservation of ovary tissues pretreated with gonadotropin-releasing hormone agonist.

Methods

In this nonclinical study performed in rats, leuprorelin acetate was administered to female Wistar rats, aged 6–8 weeks. After confirming arrest of the estrous cycle by examination of vaginal smears, ovarian tissue was cryopreserved by vitrification and slow-rate freezing prior to thawing and autotransplantation. The time required for estrous cycle recovery was assessed from vaginal smears in each group starting from day 1 of transplantation. Estradiol levels were also monitored after transplantation.

Results

The estrous cycle recovered after transplantation of ovarian tissue frozen by either method, but recovery was significantly faster after transplantation of vitrified tissue. The estradiol level also recovered by 10 days after transplantation.

Conclusions

Ovarian function was restored after transplantation of tissue preserved by either vitrification or slow-rate freezing after pretreatment with leuprorelin acetate. This method may be applicable for patients scheduled to undergo cryopreservation of ovarian tissue before chemotherapy.

Keywords

Fertility preservationGnRH analogueRatSlow freezingVitrification

Copyright information

© Japan Society for Reproductive Medicine 2010