, Volume 54, Issue 4, pp 269-274
Date: 17 May 2011

Quantitative Measurements of Xanthomonas Oryzae pv. Oryzae Distribution in Rice Using Fluorescent-Labeling

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Abstract

The rice host sensor, XA21, confers robust resistance to most strains of Xanthomonas oryzae pv. oryzae (Xoo), the casual agent of bacterial blight disease. Using in planta fluorescence imaging of Xoo strain PXO99Az expressing a green fluorescent protein (Xoo-gfp) we show that XA21 restricts Xoo spread at the point of infection. This noninvasive and quantitative method to measure spatial distribution of Xoo populations in planta facilitates detailed assessment of plant disease resistance.

K. Nozue and C.-J. Park contributed equally to this work.