Journal of Plant Biology

, 54:269

Quantitative Measurements of Xanthomonas Oryzae pv. Oryzae Distribution in Rice Using Fluorescent-Labeling

Authors

  • Kazunari Nozue
    • Department of Plant Biology, College of Biological SciencesUniversity of California, Davis
  • Chang-Jin Park
    • Department of Plant Pathology, College of Agricultural and Environmental SciencesUniversity of California, Davis
    • Department of Plant Pathology, College of Agricultural and Environmental SciencesUniversity of California, Davis
Original Research

DOI: 10.1007/s12374-011-9164-9

Cite this article as:
Nozue, K., Park, C. & Ronald, P.C. J. Plant Biol. (2011) 54: 269. doi:10.1007/s12374-011-9164-9

Abstract

The rice host sensor, XA21, confers robust resistance to most strains of Xanthomonas oryzae pv. oryzae (Xoo), the casual agent of bacterial blight disease. Using in planta fluorescence imaging of Xoo strain PXO99Az expressing a green fluorescent protein (Xoo-gfp) we show that XA21 restricts Xoo spread at the point of infection. This noninvasive and quantitative method to measure spatial distribution of Xoo populations in planta facilitates detailed assessment of plant disease resistance.

Keywords

Xanthomonas oryzae pv. oryzae, GFP, Oryza sativaXa21

Abbreviations

Xoo

Xanthomonas oryzae pv. oryzae

GFP

green fluorescent protein

CFU

colony-forming unit

Supplementary material

12374_2011_9164_Fig6_ESM.jpg (16 kb)
Fig. S1

Detection of Xoo-gfp in a Kitaake and b XA21-Kitaake immediately after smear inoculation (OD = 0.5 at 600 nm). A weak Xoo-gfp fluorescence signal was observed (upper panels), consistent with a low Xoo population in leaves. Bright field images of identical leaves are shown in lower panels. A bar indicates 1 cm. A color bar indicates Xoo-gfp concentration (CFU/mm2) based on results in Fig. 2b (JPEG 15 kb)

12374_2011_9164_MOESM1_ESM.tif (2.3 mb)
High resolution image (TIFF 2378 kb)
12374_2011_9164_Fig7_ESM.jpg (13 kb)
Fig. S1

Detection of Xoo-gfp in a Kitaake and b XA21-Kitaake immediately after smear inoculation (OD = 0.5 at 600 nm). A weak Xoo-gfp fluorescence signal was observed (upper panels), consistent with a low Xoo population in leaves. Bright field images of identical leaves are shown in lower panels. A bar indicates 1 cm. A color bar indicates Xoo-gfp concentration (CFU/mm2) based on results in Fig. 2b (JPEG 15 kb)

12374_2011_9164_MOESM2_ESM.tif (4.3 mb)
High resolution image (TIFF 4451 kb)
12374_2011_9164_Fig8_ESM.jpg (13 kb)
Fig. S1

Detection of Xoo-gfp in a Kitaake and b XA21-Kitaake immediately after smear inoculation (OD = 0.5 at 600 nm). A weak Xoo-gfp fluorescence signal was observed (upper panels), consistent with a low Xoo population in leaves. Bright field images of identical leaves are shown in lower panels. A bar indicates 1 cm. A color bar indicates Xoo-gfp concentration (CFU/mm2) based on results in Fig. 2b (JPEG 15 kb)

12374_2011_9164_MOESM3_ESM.tif (1.2 mb)
High resolution image (TIFF 1219 kb)

Copyright information

© The Botanical Society of Korea 2011