Original Research

Journal of Plant Biology

, 54:269

First online:

Quantitative Measurements of Xanthomonas Oryzae pv. Oryzae Distribution in Rice Using Fluorescent-Labeling

  • Kazunari NozueAffiliated withDepartment of Plant Biology, College of Biological Sciences, University of California, Davis
  • , Chang-Jin ParkAffiliated withDepartment of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis
  • , Pamela C. RonaldAffiliated withDepartment of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis Email author 

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Abstract

The rice host sensor, XA21, confers robust resistance to most strains of Xanthomonas oryzae pv. oryzae (Xoo), the casual agent of bacterial blight disease. Using in planta fluorescence imaging of Xoo strain PXO99Az expressing a green fluorescent protein (Xoo-gfp) we show that XA21 restricts Xoo spread at the point of infection. This noninvasive and quantitative method to measure spatial distribution of Xoo populations in planta facilitates detailed assessment of plant disease resistance.

Keywords

Xanthomonas oryzae pv. oryzae, GFP, Oryza sativa Xa21