Journal of Plant Biology

, Volume 53, Issue 2, pp 142–149

Expression Profiles of Class A Rice Heat Shock Transcription Factor Genes Under Abiotic Stresses

  • Ai-Ling Liu
  • Jie Zou
  • Xian-Wen Zhang
  • Xiao-Yun Zhou
  • Wen-Fang Wang
  • Xing-Yao Xiong
  • Li-Yun Chen
  • Xin-Bo Chen
ORIGINAL RESEARCH

DOI: 10.1007/s12374-010-9099-6

Cite this article as:
Liu, AL., Zou, J., Zhang, XW. et al. J. Plant Biol. (2010) 53: 142. doi:10.1007/s12374-010-9099-6

Abstract

Expression profiles of 12 class A rice heat shock transcription factor genes (OsHsfAs) were analyzed by semi-quantitative reverse transcriptase polymerase chain reaction. The OsHsfA genes exhibited tissue-specific expressions under normal condition. OsHsfA1a, A2d, and A9 were predominantly expressed in young spike. Expression responses of the 12 OsHsfAs under abiotic stresses were analyzed in the shoots of rice seedling. Most OsHsfA genes responded quickly to heat stress except for OsHsfA1a, A3, and A9 which were almost unaffected. In particular, OsHsfA2a expression in response to heat stress was highest among the heat shock factors examined. However, the majority of the increased OsHsfAs expression responses to salt, polyethylene glycol (PEG), and cold treatments primarily occurred during the later stages (3 to 24 h) of stress exposure. Furthermore, most of OsHsfA gene expressions were little affected and only a few (OsHsfA3, A4d, A7, and A9) genes had slow responses to cold treatment. The results indicate that the transcript levels of OsHsfAs during heat stress exposure were distinct from those of plants subjected to salt, PEG, and cold stresses, suggesting that there might be different regulatory networks between heat and non-heat stress.

Keywords

Abiotic stress Expression profile Heat shock transcription factor Oryza sativa 

Copyright information

© The Botanical Society of Korea 2010

Authors and Affiliations

  • Ai-Ling Liu
    • 1
  • Jie Zou
    • 1
  • Xian-Wen Zhang
    • 1
  • Xiao-Yun Zhou
    • 2
    • 3
  • Wen-Fang Wang
    • 1
  • Xing-Yao Xiong
    • 2
  • Li-Yun Chen
    • 4
  • Xin-Bo Chen
    • 1
    • 2
    • 3
  1. 1.College of Bioscience and BiotechnologyHunan Agricultural UniversityChangsha CityChina
  2. 2.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan ProvinceHunan Agricultural UniversityChangsha CityChina
  3. 3.Crop Gene Engineering Key Laboratory of Hunan ProvinceHunan Agricultural UniversityChangsha CityChina
  4. 4.Rice Research InstituteHunan Agricultural UniversityChangsha CityChina

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