Journal of Plant Biology

, Volume 53, Issue 2, pp 107–112

Characterization of a Stress-induced NADP-isocitrate Dehydrogenase Gene in Maize Confers Salt Tolerance in Arabidopsis

Original Research

DOI: 10.1007/s12374-009-9091-1

Cite this article as:
Liu, Y., Shi, Y., Song, Y. et al. J. Plant Biol. (2010) 53: 107. doi:10.1007/s12374-009-9091-1


In this paper, a full-length cDNA-encoding cytosolic nicotinamide adenine dinucleotide phosphate-dependent isocitrate dehydrogenase was isolated from maize and named ZmICDH. Analysis of the deduced protein sequence revealed that this gene had considerably high homologies with other plant ICDH genes such as those from rice, Arabidopsis and poplar. The gene was transcribed in all tissues tested, with the highest amount of transcript in root. Subcellular localization results indicated that ZmICDH was localized in cytosol. In an attempt to further understand the role of ZmICDH in maize, the functional characterization of the gene was conducted. The results indicated that the expression of ZmICDH was induced by drought and salt stresses. ZmICDH enhanced salt tolerance in transgenic Arabidopsis.


ZmICDHSaltDroughtStressTransgenic ArabidopsisMaize

Copyright information

© The Botanical Society of Korea 2010

Authors and Affiliations

  1. 1.Hebei North UniversityZhangjiakouHebei ProvinceChina
  2. 2.Chinese Academy of Agricultural Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)Institute of Crop ScienceBeijingChina