, Volume 11, Issue 1, pp 39-43

Transformation of sugarcane with ACC oxidase antisense gene

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The present study is an attempt to validate the function of sugarcane ACO by construction of plant expression vectors for sugarcane ACO sense and anti-sense genes and their effective transformation. Plant expression vectors for sugarcane ACO anti-sense gene was constructed and then transformed into sugarcane variety ROC22 mediated by Agrobacterium tumefaciens. The transgenic shoots were screened under the selective pressure of 40 mg/l G418 and 300 mg/l cefalexin and 19 resistant plants were obtained, two of which were further proved to be positive through detecting NPTII by PCR. The transformation rate was found 10.5%. The positive plants were found to grow slowly and were more dwarf compared to non-transgenic ones. The present work reveals the efficient genetic transformation system and development of transgenic sugarcane plant with ACO antisense gene. This work will help in understanding the ethylene and growth relationship and will also help breeders in breeding programs tailored to develop new varieties using molecular approaches.