Transformation of sugarcane with ACC oxidase antisense gene
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The present study is an attempt to validate the function of sugarcane ACO by construction of plant expression vectors for sugarcane ACO sense and anti-sense genes and their effective transformation. Plant expression vectors for sugarcane ACO anti-sense gene was constructed and then transformed into sugarcane variety ROC22 mediated by Agrobacterium tumefaciens. The transgenic shoots were screened under the selective pressure of 40 mg/l G418 and 300 mg/l cefalexin and 19 resistant plants were obtained, two of which were further proved to be positive through detecting NPTII by PCR. The transformation rate was found 10.5%. The positive plants were found to grow slowly and were more dwarf compared to non-transgenic ones. The present work reveals the efficient genetic transformation system and development of transgenic sugarcane plant with ACO antisense gene. This work will help in understanding the ethylene and growth relationship and will also help breeders in breeding programs tailored to develop new varieties using molecular approaches.
- Arencibia AD, Carmona ER, Tellez P, Chan MT, Yu SM, Trujillo LE and Oramas P (1998) An efficient protocol for sugarcane (Saccharum spp. L.) transformation of sugarcane mediated by Agrobacterium tumefaciens. Transgenic Research 7:1–10. CrossRef
- Barry CS, Llop-Tous MI, Grierson D (2000) The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiol 123(3): 979–986. CrossRef
- Edwards K, Jonestone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nuclei Acid Research 19(6):1349. CrossRef
- Elliott AR, Campbell JA, Brettell RIS, Grof CPL (1998) Agrobacterium mediated transformation of sugarcane using GFP as a screenable marker. Aust. J. Plant Physiol 25:739. CrossRef
- Obregon EGA, Padron VRI, Samsonov PDL, De La Riva GA, Housein, SG (1998) Herbicide resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium mediated transformation. Planta 206:20–27. CrossRef
- Huang YH, Tao XL, Lu L, Zhao CZ (2005) Study on the construction of a vector expressing melon ACO1 antisense gene and its transference to Nicotiana tabacum. Acta Botanica Borealioccidentalia Sinica 25(2): 262–268.
- Jiang YM, Fu JR (2000) Ethylene regulation of fruit ripening: molecular aspects. Plant Growth Regul 30:193–200. CrossRef
- Li ML, Han YF, Li L, Tian YC, Li N, Wang SJ (1999) Cloning of ACC oxidase cDNA and its inhibition of ethylene synthesis by its antisense RNA in transgenic Populus deltoids, Chinese Journal of Forest Research 12(3): 223–228.
- Li YR, Yang LT, Ye YP, Yao RL, Wang AQ, Lin YK (2007) Mechanism of ethephon on increasing cane yield and sucrose % cane in sugarcane. Southwest China Journal of Agricultural Sciences 20(1):151–156.
- Llop-Tous I, Barry CS, Grierson D (2000) Regulation of ethylene biosynthesis in response to pollination in tomato flowers. Plant Physiol 123: 971–978. CrossRef
- Luo JP, Zhang SZ, Yang BP (2003) GFP gene transformation of sugarcane mediated by Agrobacterium tumefaciens. Chinese Journal of Tropical Crop 24(4): 23–28.
- Maria GM, James EI (1993) Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment Plant Cell Reports 12:666–670.
- Wang AQ, Fan YG, Zhao XY, He LF, Yang LT, Li YR (2005) Expression of ACC oxidase gene from sugarcane induced by hormones and environmental force. Agricultural Science in China 4(8): 609–613.
- Wang GL, Fang HJ (2002) Plant Genes Engineering. Science Press, Beijing p.776–777.
- Wang ZZ, Zhang SZ, Yang BP, Li YR (2003) Trehalose Synthase gene transfer mediated by Agrobacterium tumefaciens enhances resistance to Osmotic Stress in sugarcane. Scientia Agricultura Sinica 36(2):140–146.
- Whitelaw CA, Lyssenko NN, Chen L, Zhou D, Mattoo AK, Tucker ML (2002) Delayed abscission and internodes correlate with a reduction in the ethylene receptor LeETR1 transcript in transgenic tomato. Plant Physiol 128: 978–987. CrossRef
- Wilkinson JQ, Lanahan MB, Clark DG, Bleecker AB, Chang C, Meyerowitz EM, Klee HJ (1997) A dominant mutant receptor from Arabidopsis confers ethylene insensitivity in heterologous plant. Nat. Biotechnol 15(5): 444–447. CrossRef
- Xu XF, Huang XL, Huang X (2003) Studies on the transformation of broccoli (Brassica oleracea L. var. italica) with antisense ACC oxidase gene. Acta Scientiarum Naturalium Universitatis Sunyatsen 42(4):64–68.
- Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol 35: 155–189.
- Ye ZB, Li HX, Zhou GL (1994) Genetic transformation of antisense cDNA of polygalacturonase in tomato and transgenic plant regeneration. Acta Horticulturae Sinica 21(3): 305–306.
- Transformation of sugarcane with ACC oxidase antisense gene
Volume 11, Issue 1 , pp 39-43
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- ACC oxidase
- anti-sense gene
- genetic transformation
- Industry Sectors
- Author Affiliations
- 1. Guangxi Crops Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China
- 2. Agricultural College, Guangxi University, Nanning, Guangxi, 530005, China
- 3. Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China