A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens
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- Srivastava, T., Das, S., Sopory, S.K. et al. Physiol Mol Biol Plants (2009) 15: 93. doi:10.1007/s12298-009-0010-1
Proliferation of axillary shoot buds and multiple shoot formation in Catharanthus roseus was obtained in 96 % explants on MS medium (3 % sucrose) containing NAA + BA. 2,4-D induced callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell concentration, duration of co-cultivation and acetosyringone concentration influenced transformation efficiency. Under optimal co-cultivation conditions, 98 % of the explants showed GUS expression. PCR based amplification of the transformed and subsequently selected callus tissue indicated the presence of uidA, Gly I and nptII genes.
Key wordsCatharanthus roseusMicropropagationRAPDCo-cultivationGUSGlyIAgrobacterium tumefaciensTransformation
Random amplification of polymorphic DNA
Polymerase chain reaction