Indian Journal of Clinical Biochemistry

, Volume 25, Issue 2, pp 153–157

Evaluation of plasma hormone concentrations using Enzyme-Immunoassay/Enzyme-linked Immunosorbent assay in healthy Indian men: Effect of ethnicity

  • M. Sachidhanandam
  • S. N. Singh
  • A. K. Salhan
  • Uday Sankar Ray
Original Article

DOI: 10.1007/s12291-010-0028-8

Cite this article as:
Sachidhanandam, M., Singh, S.N., Salhan, A.K. et al. Indian J Clin Biochem (2010) 25: 153. doi:10.1007/s12291-010-0028-8
  • 56 Downloads

Abstract

The study involved three ethnic groups of India; Rajputs, Gorkhas and South-Indians. Each group consisted of ∼40 healthy, male soldiers between 20–50 years. The reference ranges for cortisol, testosterone, prolactin, arginine vasopressin and proAtrial natriuretic peptide1–98 were determined using Enzyme-Immunoassay (EIA) while plasma levels of thyroid-stimulating hormone, triiodothyronine, free-triiodothyronine, thyroxine and freethyroxine were measured using Enzyme-linked immunosorbent assay (ELISA). The results indicated that plasma hormone concentrations were within physiological range and inter-ethnic differences were most prominent between north- (Rajputs and Gorkhas) and south- Indians. In comparison to Radioimmunoassay, the EIA method for prolactin, thyroid-stimulating hormone, free-thyroxine gave higher values while the ELISA method for triiodothyronine, free-triiodothyronine, and thyroxine gave lower values. These differences are due to differences in assay standards and design.

Key Words

Indian population Plasma hormones Reference intervals Ethnicity EIA ELISA 

Copyright information

© Association of Clinical Biochemists of India 2010

Authors and Affiliations

  • M. Sachidhanandam
    • 1
  • S. N. Singh
    • 1
  • A. K. Salhan
    • 1
  • Uday Sankar Ray
    • 1
    • 2
  1. 1.Defence Institute of Physiology and Allied SciencesDelhiIndia
  2. 2.Environmental PhysiologyDefence Institute of Physiology and Allied Sciences (DIPAS)Timarpur, DelhiIndia

Personalised recommendations