Breast Cancer

pp 1–9

Cytogenetic analysis of metaplastic squamous cell carcinoma of the breast inter- and intratumoral heterogeneity

  • Masahiro Oikawa
  • Akiko Igawa
  • Kenichi Taguchi
  • Kimiko Baba
  • Mayumi Ishida
  • Sayuri Akiyoshi
  • Hiroshi Yano
  • Takeshi Nagayasu
  • Shinji Ohno
  • Eriko Tokunaga
Original Article

DOI: 10.1007/s12282-017-0768-x

Cite this article as:
Oikawa, M., Igawa, A., Taguchi, K. et al. Breast Cancer (2017). doi:10.1007/s12282-017-0768-x
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Abstract

Background

Squamous cell carcinoma (SCC) of the breast is a rare and generally aggressive disease that accounts for less than 0.1% of all breast carcinomas. Although SCCs have distinct morphological features, their origin and cytogenetic profile are not well understood.

Methods

Five patients with SCC were studied. The tumor area that was predominantly composed of SCC components was macrodissected and DNA was extracted. In three cases, an invasive or noninvasive ductal carcinoma of no special type (NST) component was also present. NST-component DNA was also extracted. The tumor DNA was used for array comparative genomic hybridization analysis using a high-density oligonucleotide microarray. The cytogenetic profile of the SCC components was compared with each other and with the paired NST component in three of the five cases.

Results

The cytogenetic profile of the SCC components indicated large intertumoral heterogeneity. There were between 2 and 160 copy number alterations per case, and no common copy number alterations were identified. The cytogenetic profiles of the paired SCC and NST components were similar but not identical. Although, in one case, a larger number of copy number aberrant regions were detected in the SCC component than the NST component. In this case, all the NST component aberrations were present in the SCC component. This implies that the SCC component originated from the NST component. There were no common SCC component-specific aberrations in the three NST-component cases.

Conclusion

Our results demonstrate the cytogenetic inter- and intratumoral heterogeneity of SCC of the breast. Our comparison of cytogenetic profiles indicated that the SCC component originated from the NST component in one case.

Keywords

Breast cancer Squamous cell carcinoma Tumor heterogeneity Array CGH Pathology 

Supplementary material

12282_2017_768_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)

Copyright information

© The Japanese Breast Cancer Society 2017

Authors and Affiliations

  • Masahiro Oikawa
    • 1
    • 3
    • 4
  • Akiko Igawa
    • 1
  • Kenichi Taguchi
    • 2
  • Kimiko Baba
    • 1
  • Mayumi Ishida
    • 1
  • Sayuri Akiyoshi
    • 1
  • Hiroshi Yano
    • 4
  • Takeshi Nagayasu
    • 4
  • Shinji Ohno
    • 5
  • Eriko Tokunaga
    • 1
  1. 1.The Departments of Breast OncologyNational Kyushu Cancer CenterFukuokaJapan
  2. 2.The Departments of PathologyNational Kyushu Cancer CenterFukuokaJapan
  3. 3.The Department of Breast SurgeryNew-wa-kai Oikawa HospitalFukuokaJapan
  4. 4.The Department of Surgical OncologyNagasaki University Graduate School of Biomedical SciencesNagasakiJapan
  5. 5.The Breast Oncology CenterThe Cancer Institute Hospital of JFCRTokyoJapan