Journal of Microbiology

, Volume 53, Issue 10, pp 702–710

Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains

  • Grégoire Burgé
  • Claire Saulou-Bérion
  • Marwen Moussa
  • Florent Allais
  • Violaine Athes
  • Henry-Eric Spinnler
Article

DOI: 10.1007/s12275-015-5056-x

Cite this article as:
Burgé, G., Saulou-Bérion, C., Moussa, M. et al. J Microbiol. (2015) 53: 702. doi:10.1007/s12275-015-5056-x

Abstract

The aims of this study is to compare the growth and glucose metabolism of three Lactobacillus reuteri strains (i.e. DSM 20016, DSM 17938, and ATCC 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). However, the physiological diversity inside the species, even for basic metabolisms, like its capacity of acidification or glucose metabolism, has not been studied yet. In the present work, the growth and metabolism of three strains representative of the species diversity have been studied in batch mode. The strains were compared through characterization of growth kinetics and evaluation of acidification kinetics, substrate consumption and product formation. The results showed significant differences between the three strains which may be explained, at least in part, by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth: the phosphoketolase or heterolactic pathway (PKP) and the Embden-Meyerhof pathway (EMP). It was also shown that, in the context of obtaining a large amount of biomass, DSM 20016 and DSM 17938 strains were the most effective in terms of growth kinetics. The DSM 17938 strain, which shows the more significant metabolic shift from EMP to PKP when the pH decreases, is more effective for lactate production.

Keywords

Lactobacillus reuteri microbial growth acidification kinetics glucose metabolism Embden-Meyerhof pathway Phosphoketolase pathway lactate production 

Supplementary material

12275_2015_5056_MOESM1_ESM.pdf (153 kb)
Supplementary material, approximately 154 KB.

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Grégoire Burgé
    • 1
    • 2
    • 3
  • Claire Saulou-Bérion
    • 2
    • 3
  • Marwen Moussa
    • 2
    • 3
  • Florent Allais
    • 1
    • 2
    • 3
  • Violaine Athes
    • 2
    • 3
  • Henry-Eric Spinnler
    • 2
    • 3
  1. 1.Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTechReimsFrance
  2. 2.AgroParisTechUMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA)Thiverval-GrignonFrance
  3. 3.INRAUMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA)Thiverval-GrignonFrance

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