Journal of Microbiology

, Volume 51, Issue 2, pp 207–212

An aqueous extract of Yunnan Baiyao inhibits the quorum-sensing-related virulence of Pseudomonas aeruginosa

  • Zu-Guo Zhao
  • Shuang-Shuang Yan
  • Yun-Mei Yu
  • Na Mi
  • La-Xi Zhang
  • Jun Liu
  • Xiao-Ling Li
  • Fang Liu
  • Jun-Fa Xu
  • Wei-Qing Yang
  • Guo-Ming Li
Microbial Pathogenesis and Host-Microbe Interaction

DOI: 10.1007/s12275-013-2595-x

Cite this article as:
Zhao, ZG., Yan, SS., Yu, YM. et al. J Microbiol. (2013) 51: 207. doi:10.1007/s12275-013-2595-x

Abstract

Yunnan Baiyao is a famous Chinese medicine that has long been directly applied to wounds to reduce bleeding, pain, and swelling without causing infection. However, little is known about its ability to prevent infection. The present study aimed to assess in vitro the anti-virulence activity of an aqueous extract of Yunnan Baiyao (YBX) using Pseudomonas aeruginosa as a pathogenic model. We found that a sub-MIC (2.5 mg/ml) of YBX can efficiently interfere with the quorum-sensing (QS) signaling circuit. Real-time polymerase chain reaction analysis showed that a sub-MIC of YBX down-regulated the transcriptions of lasR, lasI, rhlR, and rhlI, which resulted in global attenuation of QS-regulated virulence activities, such as biofilm formation, and secretion of LasA protease, LasB elastase and pyocyanin. Further, YBX reduced the motility of P. aeruginosa related to QS, and impaired the formation of biofilms. These results suggest that YBX may possess global inhibitory activity against the virulence of P. aeruginosa and that YBX may also exhibit antimicrobial activity in vivo. The present study suggests that Yunnan Baiyao represents a potential source for isolating novel, safe, and efficacious antimicrobial agents.

Keywords

P. aeruginosa quorum sensing virulence motility Yunnan Baiyao 

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Zu-Guo Zhao
    • 1
  • Shuang-Shuang Yan
    • 3
  • Yun-Mei Yu
    • 2
  • Na Mi
    • 1
  • La-Xi Zhang
    • 2
  • Jun Liu
    • 1
  • Xiao-Ling Li
    • 1
  • Fang Liu
    • 1
  • Jun-Fa Xu
    • 3
  • Wei-Qing Yang
    • 1
  • Guo-Ming Li
    • 1
  1. 1.Department of Microbiology and ImmunologyGuangdong Medical CollegeZhanjiangP. R. China
  2. 2.The 422nd Central Hospitalthe People’s Liberation ArmyZhanjiangP. R. China
  3. 3.Institute of Medical LaboratoryGuangdong Medical CollegeDongguanP. R. China

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