Journal of Microbiology

, Volume 50, Issue 5, pp 785–791

Genome-wide enrichment screening reveals multiple targets and resistance genes for triclosan in Escherichia coli

Authors

  • Byung Jo Yu
    • Systems and Synthetic Biology Research CenterKorea Research of Bioscience and Biotechnology (KRIBB)
  • Jung Ae Kim
    • Systems and Synthetic Biology Research CenterKorea Research of Bioscience and Biotechnology (KRIBB)
  • Hyun Mok Ju
    • Systems and Synthetic Biology Research CenterKorea Research of Bioscience and Biotechnology (KRIBB)
  • Soo-Kyung Choi
    • Systems and Synthetic Biology Research CenterKorea Research of Bioscience and Biotechnology (KRIBB)
  • Seung Jin Hwang
    • LG Household and Healthcare Ltd
  • Sungyoo Park
    • LG Household and Healthcare Ltd
  • EuiJoong Kim
    • GenoFocus Inc.
    • Systems and Synthetic Biology Research CenterKorea Research of Bioscience and Biotechnology (KRIBB)
Article

DOI: 10.1007/s12275-012-2439-0

Cite this article as:
Yu, B.J., Kim, J.A., Ju, H.M. et al. J Microbiol. (2012) 50: 785. doi:10.1007/s12275-012-2439-0

Abstract

Triclosan is a widely used biocide effective against different microorganisms. At bactericidal concentrations, triclosan appears to affect multiple targets, while at bacteriostatic concentrations, triclosan targets FabI. The site-specific antibiotic-like mode-of-action and a widespread use of triclosan in household products claimed to possibly induce cross-resistance to other antibiotics. Thus, we set out to define more systematically the genes conferring resistance to triclosan; A genomic library of Escherichia coli strain W3110 was constructed and enriched in a selective medium containing a lethal concentration of triclosan. The genes enabling growth in the presence of triclosan were identified by using a DNA microarray and confirmed consequently by ASKA clones overexpressing the selected 62 candidate genes. Among these, forty-seven genes were further confirmed to enhance the resistance to triclosan; these genes, including the FabI target, were involved in inner or outer membrane synthesis, cell-surface material synthesis, transcriptional activation, sugar phosphotransferase (PTS) systems, various transporter systems, cell division, and ATPase and reductase/dehydrogenase reactions. In particular, overexpression of pgsA, rcsA, or gapC conferred to E. coli cells a similar level of triclosan resistance induced by fabI overexpression. These results indicate that triclosan may have multiple targets other than well-known FabI and that there are several undefined novel mechanisms for the resistance development to triclosan, thus probably inducing cross antibiotic resistance.

Keywords

triclosanmultiple targetsgenomic libraryresistanceE. coli

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012