Journal of Microbiology

, Volume 50, Issue 5, pp 813–820

The production and immunogenicity of human papillomavirus type 58 virus-like particles produced in Saccharomyces cerevisiae

  • Hye-Lim Kwag
  • Hyoung Jin Kim
  • Don Yong Chang
  • Hong-Jin Kim
Article

DOI: 10.1007/s12275-012-2292-1

Cite this article as:
Kwag, H., Kim, H.J., Chang, D.Y. et al. J Microbiol. (2012) 50: 813. doi:10.1007/s12275-012-2292-1

Abstract

Human papillomavirus (HPV) is the cause of most cases of cervical cancer. HPV type 58 (HPV58) is the second most frequent cause of cervical cancer and high-grade squamous intraepithelial lesions (HSIL) in Asia and South / Central America, respectively. However, there is no vaccine against HPV58, although there are commercially available vaccines against HPV16 and 18. In this study, we produced HPV58 L1 protein from Saccharomyces cerevisiae, and investigated its immunogenicity. We first determined the optimum period of culture for obtaining HPV58 L1. We found that a considerable portion of the HPV58 L1 resulting from 48 h culture cannot be recovered by purification, while the HPV58 L1 resulting from 144 h culture is recovered efficiently: the yield of HPV58 L1 finally recovered from 144 h culture was 2.3 times higher than that from 48 h culture, although the production level of L1 protein from 144 h culture was lower than that from 48 h culture. These results indicate that the proportion of functional L1 protein from 144 h-cultured cells is significantly higher than that of 48 h-cultured cells. The HPV58 L1 purified from the 144 h culture was correctly assembled into structures similar to naturally occurring HPV virions. Immunization with the HPV58 L1 efficiently elicited anti-HPV58 neutralizing antibodies and antigen-specific CD4+ and CD8+ T cell proliferations, without the need for adjuvant. Our findings provide a convenient method for obtaining substantial amounts of highly immunogenic HPV58 L1 from S. cerevisiae.

Keywords

human papillomavirusvaccineSaccharomyces cerevisiaevirus-like particleimmunogenicity

Supplementary material

12275_2012_2292_MOESM1_ESM.pdf (262 kb)
Supplementary material, approximately 261 KB.

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Hye-Lim Kwag
    • 1
  • Hyoung Jin Kim
    • 1
  • Don Yong Chang
    • 1
  • Hong-Jin Kim
    • 1
  1. 1.College of PharmacyChung-Ang UniversitySeoulRepublic of Korea