Journal of Microbiology

, Volume 50, Issue 4, pp 693–697

Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli

Authors

  • Qi Wang
    • Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences
    • Graduate University of Chinese Academy of Sciences
  • Changshui Liu
    • Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences
    • Graduate University of Chinese Academy of Sciences
  • Mo Xian
    • Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences
  • Yongguang Zhang
    • Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences
    • Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences
Note

DOI: 10.1007/s12275-012-2234-y

Cite this article as:
Wang, Q., Liu, C., Xian, M. et al. J Microbiol. (2012) 50: 693. doi:10.1007/s12275-012-2234-y

Abstract

Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexusaurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.

Keywords

poly(3-hydroxypropionate)malonyl-CoA reductasepropionyl-coenzyme A synthetasepolyhydroxyalkanoate synthaserecombinant E. coli
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© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012