The Journal of Microbiology

, Volume 50, Issue 2, pp 311–319

Porphyromonas gingivalis-derived lipopolysaccharide-mediated activation of MAPK signaling regulates inflammatory response and differentiation in human periodontal ligament fibroblasts

  • Taegun Seo
  • Seho Cha
  • Tae-Il Kim
  • Jeong-Soon Lee
  • Kyung Mi Woo
Articles

DOI: 10.1007/s12275-012-2146-x

Cite this article as:
Seo, T., Cha, S., Kim, T. et al. J Microbiol. (2012) 50: 311. doi:10.1007/s12275-012-2146-x
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Abstract

Porphyromonas gingivalis (P.g.), which is a potential pathogen for periodontal diseases, contains lipopolysaccharide (LPS), and this endotoxin stimulates a variety of cellular responses. At present, P.g.-derived LPS-induced cellular responses in human periodontal ligament fibroblasts (PDLFs) are not well characterized. Here, we demonstrate that P.g-derived LPS regulates inflammatory responses, apoptosis and differentiation in PDLFs. Interleukin-6 (IL-6) and -8 (IL-8) were effectively upregulated by treatment of P.g.-derived LPS, and we confirmed apoptosis markers including elevated cytochrome c levels, active caspase-3 and morphological change in the presence of P.g.-derived LPS. Moreover, when PDLFs were cultured with differentiation media, P.g.-derived LPS reduced the expression of differentiation marker genes, as well as reducing alkaline phosphatase (ALP) activity and mineralization. P.g.-derived LPS-mediated these cellular responses were effectively abolished by treatment of mitogen-activated protein kinase (MAPK) inhibitors. Taken together, our results suggest that P.g.-derived LPS regulates several cellular responses via activation of MAPK signaling pathways in PDLFs.

Keywords

apoptosiscell differentiationlipopolysaccharidemitogen-activated protein kinaseperiodontal ligamentPorphyromonas gingivalis

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Taegun Seo
    • 1
  • Seho Cha
    • 1
  • Tae-Il Kim
    • 2
  • Jeong-Soon Lee
    • 2
  • Kyung Mi Woo
    • 3
  1. 1.Department of Life ScienceDongguk University-SeoulSeoulRepublic of Korea
  2. 2.Department of Periodontology, Dental Research InstituteSeoul National University School of DentistrySeoulRepublic of Korea
  3. 3.Department of Cell and Developmental BiologySeoul National University School of DentistrySeoulRepublic of Korea