Articles

The Journal of Microbiology

, Volume 50, Issue 2, pp 311-319

First online:

Porphyromonas gingivalis-derived lipopolysaccharide-mediated activation of MAPK signaling regulates inflammatory response and differentiation in human periodontal ligament fibroblasts

  • Taegun SeoAffiliated withDepartment of Life Science, Dongguk University-Seoul
  • , Seho ChaAffiliated withDepartment of Life Science, Dongguk University-Seoul
  • , Tae-Il KimAffiliated withDepartment of Periodontology, Dental Research Institute, Seoul National University School of Dentistry Email author 
  • , Jeong-Soon LeeAffiliated withDepartment of Periodontology, Dental Research Institute, Seoul National University School of Dentistry
  • , Kyung Mi WooAffiliated withDepartment of Cell and Developmental Biology, Seoul National University School of Dentistry

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Abstract

Porphyromonas gingivalis (P.g.), which is a potential pathogen for periodontal diseases, contains lipopolysaccharide (LPS), and this endotoxin stimulates a variety of cellular responses. At present, P.g.-derived LPS-induced cellular responses in human periodontal ligament fibroblasts (PDLFs) are not well characterized. Here, we demonstrate that P.g-derived LPS regulates inflammatory responses, apoptosis and differentiation in PDLFs. Interleukin-6 (IL-6) and -8 (IL-8) were effectively upregulated by treatment of P.g.-derived LPS, and we confirmed apoptosis markers including elevated cytochrome c levels, active caspase-3 and morphological change in the presence of P.g.-derived LPS. Moreover, when PDLFs were cultured with differentiation media, P.g.-derived LPS reduced the expression of differentiation marker genes, as well as reducing alkaline phosphatase (ALP) activity and mineralization. P.g.-derived LPS-mediated these cellular responses were effectively abolished by treatment of mitogen-activated protein kinase (MAPK) inhibitors. Taken together, our results suggest that P.g.-derived LPS regulates several cellular responses via activation of MAPK signaling pathways in PDLFs.

Keywords

apoptosis cell differentiation lipopolysaccharide mitogen-activated protein kinase periodontal ligament Porphyromonas gingivalis