The Journal of Microbiology

, Volume 49, Issue 5, pp 834–840

Berberine inhibits HEp-2 cell invasion induced by Chlamydophila pneumoniae infection

  • Li Jun Zhang
  • Li Jun Zhang
  • Wei Quan
  • Bei Bei Wang
  • Bing Ling Shen
  • Teng Teng Zhang
  • Yi Kang
Articles

DOI: 10.1007/s12275-011-1051-z

Cite this article as:
Zhang, L.J., Zhang, L.J., Quan, W. et al. J Microbiol. (2011) 49: 834. doi:10.1007/s12275-011-1051-z

Abstract

This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.

Keywords

berberine Chlamydia pneumoniae cell invasion cell adhesion cell migration 

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg  2011

Authors and Affiliations

  • Li Jun Zhang
    • 1
  • Li Jun Zhang
    • 1
  • Wei Quan
    • 1
  • Bei Bei Wang
    • 1
  • Bing Ling Shen
    • 1
  • Teng Teng Zhang
    • 1
  • Yi Kang
    • 2
  1. 1.Department of PathophysiologyBasic Medical School of Tianjin Medical UniversityTianjinP. R. China
  2. 2.Department of PharmacologyBasic Medical School of Tianjin Medical UniversityTianjinP. R. China

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