The Journal of Microbiology

, Volume 48, Issue 6, pp 872–876

Analysis of cytoplasmic membrane proteome of Streptococcus pneumoniae by shotgun proteomic approach

  • Chi-Won Choi
  • Sung-Ho Yun
  • Sang-Oh Kwon
  • Sun-Hee Leem
  • Jong-Soon Choi
  • Chi-Young Yun
  • Seung Il Kim
Note

DOI: 10.1007/s12275-010-0220-9

Cite this article as:
Choi, CW., Yun, SH., Kwon, SO. et al. J Microbiol. (2010) 48: 872. doi:10.1007/s12275-010-0220-9

Abstract

In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferse systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.

Keywords

Streptococcus pneumoniae membrane proteome 1-DE/LC/MS-MS 

Supplementary material

12275_2010_220_MOESM1_ESM.pdf (96 kb)
Supplementary material, approximately 95.7 KB.

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Chi-Won Choi
    • 1
    • 2
  • Sung-Ho Yun
    • 1
  • Sang-Oh Kwon
    • 1
    • 3
  • Sun-Hee Leem
    • 4
  • Jong-Soon Choi
    • 1
    • 3
  • Chi-Young Yun
    • 2
  • Seung Il Kim
    • 1
  1. 1.Devision of Life ScienceKorea Basic Science InstituteDaejeonRepublic of Korea
  2. 2.Department of BiologyDaejeon UniversityDaejeonRepublic of Korea
  3. 3.Graduate School of Analytical Science and TechnologyChungnam National UniversityDaejeonRepublic of Korea
  4. 4.Department of Biology and Biomedical ScienceDong-A UniversityBusanRepublic of Korea