The Journal of Microbiology

, Volume 48, Issue 5, pp 594–600

Effects of crude oil on marine microbial communities in short term outdoor microcosms

Authors

  • Seung Won Jung
    • South Sea Environment Research DepartmentKorea Ocean Research & Development Institute
  • Joon Sang Park
    • Department of BiologySangmyung University
  • Oh Youn Kown
    • South Sea Environment Research DepartmentKorea Ocean Research & Development Institute
  • Jung-Hoon Kang
    • South Sea Environment Research DepartmentKorea Ocean Research & Development Institute
  • Won Joon Shim
    • Oil and POPs Research GroupKorea Ocean Research & Development Institute
    • South Sea Environment Research DepartmentKorea Ocean Research & Development Institute
Article

DOI: 10.1007/s12275-010-0199-2

Cite this article as:
Jung, S.W., Park, J.S., Kown, O.Y. et al. J Microbiol. (2010) 48: 594. doi:10.1007/s12275-010-0199-2

Abstract

To assess the effects of crude oil spills on marine microbial communities, 10 L outdoor microcosms were manipulated over an exposure period of 8 days. The responses of microbial organisms exposed to five crude oil concentrations in 10 to 10,000 ppm (v/v) were monitored in the microcosms. The abundance of microalgae and copepods decreased rapidly upon the addition of crude oil at concentrations over 1,000 ppm, whereas the total density of heterotrophic bacteria increased dramatically at the higher concentrations. Bacterial diversity, determined by denaturing gradient gel electrophoresis, was increased at higher concentrations. In particular, the intensity of the bands representing Jannaschia sp. and Sulfitobacter brevis increased with the addition of oil. These results indicate that crude oil spills with concentrations over 1,000 ppm seriously affected the structure of the microbial communities.

Keywords

DGGEmicrobial communitiesmicrocosmoil spillspollution effectsrisk assessment
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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2010