The Journal of Microbiology

, Volume 48, Issue 5, pp 594–600

Effects of crude oil on marine microbial communities in short term outdoor microcosms

  • Seung Won Jung
  • Joon Sang Park
  • Oh Youn Kown
  • Jung-Hoon Kang
  • Won Joon Shim
  • Young-Ok Kim
Article

DOI: 10.1007/s12275-010-0199-2

Cite this article as:
Jung, S.W., Park, J.S., Kown, O.Y. et al. J Microbiol. (2010) 48: 594. doi:10.1007/s12275-010-0199-2

Abstract

To assess the effects of crude oil spills on marine microbial communities, 10 L outdoor microcosms were manipulated over an exposure period of 8 days. The responses of microbial organisms exposed to five crude oil concentrations in 10 to 10,000 ppm (v/v) were monitored in the microcosms. The abundance of microalgae and copepods decreased rapidly upon the addition of crude oil at concentrations over 1,000 ppm, whereas the total density of heterotrophic bacteria increased dramatically at the higher concentrations. Bacterial diversity, determined by denaturing gradient gel electrophoresis, was increased at higher concentrations. In particular, the intensity of the bands representing Jannaschia sp. and Sulfitobacter brevis increased with the addition of oil. These results indicate that crude oil spills with concentrations over 1,000 ppm seriously affected the structure of the microbial communities.

Keywords

DGGEmicrobial communitiesmicrocosmoil spillspollution effectsrisk assessment

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Seung Won Jung
    • 1
  • Joon Sang Park
    • 2
  • Oh Youn Kown
    • 1
  • Jung-Hoon Kang
    • 1
  • Won Joon Shim
    • 3
  • Young-Ok Kim
    • 1
  1. 1.South Sea Environment Research DepartmentKorea Ocean Research & Development InstituteGeojeRepublic of Korea
  2. 2.Department of BiologySangmyung UniversitySeoulRepublic of Korea
  3. 3.Oil and POPs Research GroupKorea Ocean Research & Development InstituteGeojeRepublic of Korea