The Journal of Microbiology

, Volume 46, Issue 6, pp 737–743

Protective effect of polygoni cuspidati radix and emodin on Vibrio vulnificus cytotoxicity and infection

Authors

  • Jong Ro Kim
    • Department of Oriental Medicine MaterialsDongshin University
    • Clinical Vaccine R&D CenterChonnam National University Medical School
  • Dool-Ri Oh
    • Department of Oriental Medicine MaterialsDongshin University
    • Clinical Vaccine R&D CenterChonnam National University Medical School
  • Mi Hye Cha
    • Department of Oriental Medicine MaterialsDongshin University
    • Clinical Vaccine R&D CenterChonnam National University Medical School
  • Byoung Sik Pyo
    • Department of Oriental Medicine MaterialsDongshin University
  • Joon Haeng Rhee
    • Clinical Vaccine R&D CenterChonnam National University Medical School
    • National Research Laboratory of Molecular Microbial PathogenesisChonnam National University Medical School
    • Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic BacteriaChonnam National University Medical School
  • Hyon E. Choy
    • Clinical Vaccine R&D CenterChonnam National University Medical School
    • Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic BacteriaChonnam National University Medical School
  • Won Keun Oh
    • College of PharmacyChosun University
    • Department of Oriental Medicine MaterialsDongshin University
    • Clinical Vaccine R&D CenterChonnam National University Medical School
Article

DOI: 10.1007/s12275-008-0232-x

Cite this article as:
Kim, J.R., Oh, D., Cha, M.H. et al. J Microbiol. (2008) 46: 737. doi:10.1007/s12275-008-0232-x

Abstract

Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.

Keywords

V. vulnificus infectionemodinPolygoni Cuspidati Radixcytotoxicityantibacterial activity
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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008