Response of Saccharomyces cerevisiae to stress-free acidification
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Genome-wide transcriptional analysis of a Saccharomyces cerevisiae batch culture revealed that more than 829 genes were regulated in response to an environmental shift from pH 6 to pH 3 by added sulfuric acid. This shift in pH was not detrimental to the rate of growth compared to a control culture that was maintained at pH 6 and the transcriptional changes most strikingly implicated not up- but down-regulation of stress responses. In addition, the transcriptional changes upon acid addition indicated remodeling of the cell wall and central carbon metabolish. The overall trend of changes was similar for the pH-shift experiment and the pH 6 control. However, the changes in the pH 6 control were much weaker and occurred 2.5 h later than in the pH-shift experiment. Thus, the reaction to the steep pH decrease was an immediate response within the normal repertoire of adaptation shown in later stages of fermentation at pH 6. Artificially preventing the yeast from acidifying the medium may be considered physiologically stressful under the tested conditions.
- Blank, L.M. and U. Sauer. 2004. TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates. Microbiology 150, 1085–1093. CrossRef
- Boorsma, A., H. De Nobel, B. Ter Riet, B. Bargmann, S. Brul, K.J. Hellingwerf, and F.M. Klis. 2004. Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae. Yeast 21, 413–427. CrossRef
- Causton, H.C., B. Ren, S.S. Koh, C.T. Harbison, E. Kanin, E.G. Jennings, T.I. Lee, H.L. True, E.S. Lander, and R.A. Young. 2001. Remodeling of yeast genome expression in response to environmental changes. Mol. Biol. Cell. 12, 323–337.
- Chen, A.K., M. Breuer, B. Hauer, P.L. Rogers, and B. Rosche. 2005. pH shift enhancement of Candida utilis pyruvate decarboxylase production. Biotechnol. Bioeng. 92, 183–188. CrossRef
- Eisen, M.B., P.T. Spellman, P.O. Brown, and D. Botstein. 1998. Cluster analysis and display of genome-wide expression patterns. Proc. Natl. Acad. Sci. USA 95, 14863–14868. CrossRef
- Gasch, A.P., P.T. Spellman, C.M. Kao, O. Carmel-Harel, M.B. Eisen, G. Storz, D. Botstein, and P.O. Brown. 2000. Genomic expression programs in the response of yeast cells to environmental changes. Mol. Biol. Cell. 11, 4241–4257.
- Gelling, C.L., M.D. Piper, S.P. Hong, G.D. Kornfeld, and I.W. Dawes. 2004. Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiae. J. Biol. Chem. 279, 7072–7081. CrossRef
- Hatzixanthis, K., M. Mollapour, I. Seymour, B.E. Bauer, G. Krapf, C. Schuller, K. Kuchler, and P.W. Piper. 2003. Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP-binding cassette transporter. Yeast 20, 575–585. CrossRef
- Kapteyn, J.C., B. Ter Riet, E. Vink, S. Blad, H. De Nobel, H. Van Den Ende, and F.M. Klis. 2001. Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall. Mol. Microbiol. 39, 469–479. CrossRef
- Kawahata, M., K. Masaki, T. Fujii, and H. Iefuji. 2006. Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p. FEMS Yeast Res. 6, 924–936. CrossRef
- Lawrence, C.L., C.H. Botting, R. Antrobus, and P.J. Coote. 2004. Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress. Mol. Cell. Biol. 24, 3307–3323. CrossRef
- Piper, P., C.O. Calderon, K. Hatzixanthis, and M. Mollapour. 2001. Weak acid adaptation: the stress response that confers yeasts with resistance to organic acid food preservatives. Microbiology 147, 2635–2642.
- Robinson, M.D., J. Grigull, N. Mohammad, and T.R. Hughes. 2002. FunSpec: a web-based cluster interpreter for yeast. BMC Bioinformatics 3, 35. CrossRef
- Rosche, B., N. Leksawasdi, V. Sandford, M. Breuer, B. Hauer, and P. Rogers. 2002. Enzymatic (R)-phenylacetylcarbinol production in benzaldehyde emulsions. Appl. Microbiol. Biotechnol. 60, 94–100. CrossRef
- Schmitt, M.E., T.A. Brown, and B.L. Trumpower. 1990. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18, 3091–3092. CrossRef
- Schuller, C., Y.M. Mamnun, M. Mollapour, G. Krapf, M. Schuster, B.E. Bauer, P.W. Piper, and K. Kuchler. 2004. Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae. Mol. Biol. Cell. 15, 706–720. CrossRef
- Serrano, R., A. Ruiz, D. Bernal, J.R. Chambers, and J. Arino. 2002. The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol. Microbiol. 46, 1319–1333. CrossRef
- Sigler, K. and M. Hofer. 1991. Mechanisms of acid extrusion in yeast. Biochim. Biophys. Acta. 1071, 375–391.
- Warner, J. 1999. The economics of ribosome biosynthesis in yeast. Trends Biochem. Sci. 24, 437–440. CrossRef
- Response of Saccharomyces cerevisiae to stress-free acidification
The Journal of Microbiology
Volume 47, Issue 1 , pp 1-8
- Cover Date
- Print ISSN
- Online ISSN
- The Microbiological Society of Korea
- Additional Links
- stress response
- Saccharomyces cerevisiae
- pyruvate decarboxylase
- Industry Sectors
- Author Affiliations
- 1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia
- 2. Ramaciotti Centre for Gene Function Analysis, University of New South Wales, Sydney, NSW, 2052, Australia